Bianchi A T, Zwart R J, Jeurissen S H, Moonen-Leusen H W
Central Veterinary Institute, Lelystad, Netherlands.
Vet Immunol Immunopathol. 1992 Aug;33(3):201-21. doi: 10.1016/0165-2427(92)90182-p.
Using immunohistological techniques, we studied the development over time of B- and T-cell compartments in the lymphoid organs of specific-pathogen-free pigs. Tissue samples were collected at various time-points, starting 2 days before the pigs were born until the pigs were 10 months old. The samples were collected from the spleen, thymus, peripheral lymph node, mesenteric lymph node, duodenum, jejunum, ileum, jejunal Peyer's patch and ileal Peyer's patch. Monoclonal antibodies specific to B- and T-cells were used to identify where the following cells were localized: IgM-B cells (cells positive to surface immunoglobulin), IgM-, IgG- and IgA-containing cells (cells positive to cytoplasmic immunoglobulin), and CD2-, CD4- and CD8-positive cells. The development of the B- and T-cell subpopulations in each organ was analysed. Two days before birth, most organs contained quantities of IgM-B cells. The spleen, lymph nodes, Peyer's patches and, notably, the thymus, contained some immunoglobulin-containing cells (Ig-CC); this finding indicates that pigs have cells that secrete immunoglobulins before birth. Just after birth, the incidence of Ig-CC increased in most organs; first IgM-CC increased, then either IgG- or IgA-CC increased, depending on the organ. T-cell development was observed clearly in spleen and in the lamina propria of the small intestine, in contrast to other organs, in which the T-cell compartments containing various T-cell subpopulations were well developed before birth. Comparison of the incidence of CD4+ and CD8+ cells showed that the CD4:CD8 ratio of these cells in the spleen, lymph nodes, Peyer's patches and small intestine is low, especially in adult pigs, compared with the CD4:CD8 ratio in other species. Weaning had little influence on the incidence of B- and T-cells in lymphoid organs. This study is the first immunohistological survey to describe the development of the major B- and T-cell subpopulations in various lymphoid organs of pigs, and it should be useful for future immunopathological and comparative immunological studies in pigs.
我们运用免疫组织学技术，研究了无特定病原体猪淋巴器官中B细胞和T细胞区室随时间的发育情况。在猪出生前2天至10月龄的不同时间点采集组织样本。样本取自脾脏、胸腺、外周淋巴结、肠系膜淋巴结、十二指肠、空肠、回肠、空肠派伊尔结和回肠派伊尔结。使用针对B细胞和T细胞的单克隆抗体来确定以下细胞的定位：IgM - B细胞（表面免疫球蛋白阳性细胞）、含IgM、IgG和IgA的细胞（胞质免疫球蛋白阳性细胞）以及CD2、CD4和CD8阳性细胞。分析了各器官中B细胞和T细胞亚群的发育情况。出生前2天，大多数器官含有大量IgM - B细胞。脾脏、淋巴结、派伊尔结，尤其是胸腺，含有一些含免疫球蛋白的细胞（Ig - CC）；这一发现表明猪在出生前就有分泌免疫球蛋白的细胞。出生后不久，大多数器官中Ig - CC的发生率增加；首先是IgM - CC增加，然后根据器官不同，IgG - CC或IgA - CC增加。与其他器官不同，在脾脏和小肠固有层中可清楚观察到T细胞的发育，其他器官中含有各种T细胞亚群的T细胞区室在出生前就已发育良好。CD4 +和CD8 +细胞发生率的比较表明，与其他物种相比，脾脏、淋巴结、派伊尔结和小肠中这些细胞的CD4:CD8比值较低，尤其是成年猪。断奶对淋巴器官中B细胞和T细胞的发生率影响很小。本研究是首次通过免疫组织学调查描述猪各种淋巴器官中主要B细胞和T细胞亚群的发育情况，对未来猪的免疫病理学和比较免疫学研究应具有一定价值。
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