Betto P, Meneguz A, Ricciarello G, Pichini S
Istituto Superiore di Sanità, Rome, Italy.
J Chromatogr. 1992 Mar 13;575(1):117-21. doi: 10.1016/0378-4347(92)80511-n.
A selective and sensitive high-performance liquid chromatographic method with coulometric detection is described for the quantitation of buspirone and its active metabolite, 1-(2-pyrimidinyl)piperazine, in plasma samples of mice treated orally with buspirone (10 mg/kg body weight). The analytes are extracted with a carboxylic acid solid-phase extraction column before chromatography. A dual-electrode electrochemical detector is used. The limit of detection is 50 pg for buspirone and 35 pg for 1-(2-pyrimidinyl)piperazine.
描述了一种采用库仑检测的选择性灵敏高效液相色谱法，用于定量测定口服给予丁螺环酮（10 mg/kg体重）的小鼠血浆样品中的丁螺环酮及其活性代谢物1-(2-嘧啶基)哌嗪。分析物在色谱分析前用羧酸固相萃取柱进行萃取。使用双电极电化学检测器。丁螺环酮的检测限为50 pg，1-(2-嘧啶基)哌嗪的检测限为35 pg。
