SCANU A, LEWIS L A, PAGE I H
J Exp Med. 1958 Aug 1;108(2):185-96. doi: 10.1084/jem.108.2.185.
The techniques of agar immunoelectrophoresis and agar double diffusion were applied to the study of the antigenicity of beta- and alpha(1)-lipoproteins separated by ultracentrifugation from normal human sera. The effects of delipidation were also investigated. It was shown that beta- and alpha(1)-lipoproteins are antigenically distinct. For each class of lipoprotein studied, a single antigenic component was demonstrated. In some, but not all, preparations of alpha(1)-lipoprotein a second, small antigenic component was detected, and identified as albumin. Absorption with lipoprotein-free serum or albumin removed this component without changing the lipoprotein band. Delipidation did not affect the antigenicity of either beta- or alpha(1)-lipoproteins. Immunoelectrophoresis, because of its high sensitivity and specificity, provides an additional criterion of purity for antigenic proteins in addition to the data that can be obtained from ultracentrifugal and free electrophoretic analysis.
采用琼脂免疫电泳和琼脂双向扩散技术，研究从正常人血清中通过超速离心分离出的β-脂蛋白和α(1)-脂蛋白的抗原性。同时也研究了脱脂的影响。结果表明，β-脂蛋白和α(1)-脂蛋白在抗原性上是不同的。对于所研究的每一类脂蛋白，均显示出单一的抗原成分。在部分（但并非全部）α(1)-脂蛋白制剂中，检测到第二种小的抗原成分，并鉴定为白蛋白。用无脂蛋白血清或白蛋白吸收可去除该成分，而不改变脂蛋白条带。脱脂并不影响β-脂蛋白或α(1)-脂蛋白的抗原性。免疫电泳由于其高灵敏度和特异性，除了可从超速离心和自由电泳分析获得的数据外，还为抗原性蛋白质提供了纯度的额外标准。
