DELALLA L, LEVINE L, BROWN R K
J Exp Med. 1957 Aug 1;106(2):261-71. doi: 10.1084/jem.106.2.261.
High density serum lipoprotein underwent serologic and physicochemical alterations on aging during storage at 0 degrees C. for 1 month, as judged by decrease of diffusion coefficient and increase of C' fixation. Ultracentrifugation, dialysis, and high concentrations of sodium chloride did not cause these changes. A protein sedimenting at density 1.24 in the ultracentrifuge reacted with antiserum to high density lipoprotein. Probably it was the protein portion of alpha lipoprotein dissociated from the lipide during ultracentrifugation. Although the antiserum to high density lipoprotein did not react with low density lipoprotein prepared from normal serum, it reacted with similarly prepared lipoproteins from the serum of a patient with biliary cirrhosis.
在0℃储存1个月期间，高密度血清脂蛋白在老化过程中发生了血清学和物理化学改变，这可通过扩散系数降低和补体（C'）结合增加来判断。超速离心、透析和高浓度氯化钠不会引起这些变化。在超速离心机中密度为1.24时沉淀的一种蛋白质与抗高密度脂蛋白抗血清发生反应。它可能是在超速离心过程中从脂质中解离出来的α脂蛋白的蛋白质部分。尽管抗高密度脂蛋白抗血清不与从正常血清制备的低密度脂蛋白发生反应，但它与从一位胆汁性肝硬化患者血清中以类似方式制备的脂蛋白发生反应。
