Silverman L M, Gruemer H D
Clin Chem. 1976 Oct;22(10):1710-4.
We report specific findings in the imipramine/serotonin animal model that are consistent with sarcolemmal membrane alterations. Among these findings are cytoplasmic enzyme release, diminished uptake of alpha-aminoisobutyrate (an amino acid analog), decreased oxygen consumption in isolated rat diaphragm, and ribosuria. Furthermore, we describe for the first time the release of the MB isoenzyme of creatine kinase from a source other than cardiac tissue; that is, isolated diaphragms from imipramine/serotonin-treated animals release increased amounts of MB isoenzyme as compared to diaphragms from control animals. We believe the similarities between this animal model and the human disease (Duchenne muscular dystrophy) support a genetically determined generalized membrane abnormality in the pathogenesis of this form of muscular dystrophy.
我们报告了丙咪嗪/血清素动物模型中的特定发现，这些发现与肌膜改变一致。这些发现包括细胞质酶释放、α-氨基异丁酸（一种氨基酸类似物）摄取减少、离体大鼠膈肌耗氧量降低以及核糖尿。此外，我们首次描述了肌酸激酶MB同工酶从心脏组织以外的来源释放；也就是说，与对照动物的膈肌相比，用丙咪嗪/血清素处理的动物的离体膈肌释放出更多的MB同工酶。我们认为这种动物模型与人类疾病（杜氏肌营养不良症）之间的相似性支持了这种形式的肌营养不良症发病机制中存在一种由基因决定的全身性膜异常。
