蛇（巴西矛头蝮）体内循环的血管紧张素。

Circulating vasotocin in the snake Bothrops jararaca.

Silveira P F, Schiripa L N, Carmona E, Picarelli Z P

Serviço de Farmacologia, Instituto Butantan, São Paulo, Brazil.

Comp Biochem Physiol Comp Physiol. 1992 Sep;103(1):59-64. doi: 10.1016/0300-9629(92)90241-h.

Abstract

There is biochemical and pharmacological evidence to suggest the presence of vasotocin in the blood and plasma of the snake Bothrops jararaca (Bj). 2. XE-64 extracts from Bj blood showed antidiuretic and hypotensive activities in rats and a contractile effect on rat isolated uterus, which was totally dialysable and inhibited by thioglycollate. 3. Extracts from Bj whole plasma presented an antidiuretic activity which was only partially dialysable. 4. The plasma extracts also showed oxytocic properties. 5. When EDTA and Sep-Pak C18 extraction were used, a better recovery and characterization of vasotocin by HPLC was obtained. 6. These results indicate the occurrence of free and bound circulating vasotocin in Bj, in an equilibrium dependent of its enzymatic hydrolysis.

摘要

有生化和药理学证据表明，在巴西矛头蝮蛇（Bj）的血液和血浆中存在加压催产素。2. 从Bj血液中提取的XE - 64在大鼠体内显示出抗利尿和降压活性，对大鼠离体子宫有收缩作用，该作用完全可透析且受巯基乙酸抑制。3. Bj全血浆提取物呈现出抗利尿活性，且仅部分可透析。4. 血浆提取物还表现出催产素特性。5. 当使用EDTA和Sep - Pak C18萃取时，通过高效液相色谱法能更好地回收和鉴定加压催产素。6. 这些结果表明，在Bj中存在游离和结合形式的循环加压催产素，其处于依赖酶促水解的平衡状态。