Schmid K W, Kirchmair R, Ladurner D, Fischer-Colbrie R, Böcker W
Department of Pathology, University of Münster, Germany.
Histopathology. 1992 Sep;21(3):225-32. doi: 10.1111/j.1365-2559.1992.tb00380.x.
Normal and hyperplastic thyroid C-cells and 14 cases of medullary thyroid carcinoma were investigated immunohistochemically with antibodies against chromogranins A and B, secretogranin II, calcitonin and calcitonin gene-related peptide (CGRP). Normal and hyperplastic C-cells showed strong calcitonin and chromogranin A immunoreactivity whereas CGRP, chromogranin B and secretogranin II expression was less intense. Strong calcitonin and chromogranin A immunoreactivity was also found in the majority of tumour cells in medullary thyroid carcinoma. The CGRP, chromogranin B and secretogranin II staining observed was present in variable patterns. In some cases CGRP, chromogranin B and secretogranin II could only be demonstrated in isolated tumour cells with elongated processes suggestive of neuronal differentiation of these cells. The biological function(s) of the chromogranins/secretogranins remain(s) still unclear. There is evidence that these proteins are pro-peptides which give rise to functionally active compounds. Studies on normal C-cells and medullary thyroid carcinoma may elucidate the role of chromogranins/secretogranins in endocrine and neuronal cells.
采用抗嗜铬粒蛋白A和B、分泌粒蛋白II、降钙素及降钙素基因相关肽（CGRP）的抗体，对正常和增生性甲状腺C细胞以及14例甲状腺髓样癌进行免疫组织化学研究。正常和增生性C细胞显示出强烈的降钙素和嗜铬粒蛋白A免疫反应性，而CGRP、嗜铬粒蛋白B和分泌粒蛋白II的表达则较弱。在大多数甲状腺髓样癌的肿瘤细胞中也发现了强烈的降钙素和嗜铬粒蛋白A免疫反应性。观察到的CGRP、嗜铬粒蛋白B和分泌粒蛋白II染色呈现出不同的模式。在某些情况下，CGRP、嗜铬粒蛋白B和分泌粒蛋白II仅在具有细长突起的孤立肿瘤细胞中得以证实，提示这些细胞具有神经元分化。嗜铬粒蛋白/分泌粒蛋白的生物学功能仍不清楚。有证据表明这些蛋白质是前体肽，可产生具有功能活性的化合物。对正常C细胞和甲状腺髓样癌的研究可能会阐明嗜铬粒蛋白/分泌粒蛋白在内分泌和神经元细胞中的作用。
