Schmid K W, Fischer-Colbrie R, Hagn C, Jasani B, Williams E D, Winkler H
Am J Surg Pathol. 1987 Jul;11(7):551-6. doi: 10.1097/00000478-198707000-00007.
We have studied the presence of chromogranins A and B and secretogranin II in medullary carcinomas of the thyroid using the methods of immunoblotting and immunohistochemistry. All three antigens were identified by immunoblotting. Chromogranin A and secretogranin II behaved identically to the adrenal antigens in electrophoresis, whereas the molecular size of chromogranin B in the thyroid tissue appeared slightly smaller, possibly due to a higher degree of proteolytic processing. In immunohistochemistry, positive staining was obtained with all three antigens throughout the tumor tissue. All three antigens, as well as the hormone calcitonin, were apparently present in the same cells. Our study suggests that in addition to chromogranin A, the other two antigens (chromogranin B and secretogranin II) may also be useful markers for tumors arising from endocrine tissues.
我们运用免疫印迹法和免疫组织化学方法，研究了甲状腺髓样癌中嗜铬粒蛋白A和B以及分泌粒蛋白II的存在情况。通过免疫印迹法鉴定出了所有这三种抗原。嗜铬粒蛋白A和分泌粒蛋白II在电泳中表现与肾上腺抗原相同，而甲状腺组织中嗜铬粒蛋白B的分子大小似乎略小，这可能是由于蛋白水解加工程度较高所致。在免疫组织化学中，这三种抗原在整个肿瘤组织中均呈现阳性染色。所有这三种抗原以及激素降钙素显然存在于同一细胞中。我们的研究表明，除嗜铬粒蛋白A外，其他两种抗原（嗜铬粒蛋白B和分泌粒蛋白II）也可能是内分泌组织来源肿瘤的有用标志物。
