Okada H, Toyota N, Harimaya Y, Matsukawa K
Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
J Comp Pathol. 1991 Oct;105(3):263-70. doi: 10.1016/s0021-9975(08)80194-6.
Hyperplasia and hypertrophy of C cells were demonstrated in sheep with hypercalcaemia induced by administration of vitamin D3 (2 million I.U. per day). After treatment with vitamin D3 for 10, 20 or 30 days, serum calcium values increased to 10.28, 11.86 and 10.44 mg per dl, respectively, compared to a normal concentration of around 9 mg per dl. Immunohistochemical reactions of calcitonin, chromogranin A and calcitonin gene-related peptide (CGRP) decreased, whereas intense neurone-specific enolase (NSE) immunoreactivity was noted in C cells. Immunohistochemical staining with anti-calcitonin, anti-chromogranin A, anti-CGRP and anti-NSE antisera was useful to demonstrate the functional state of stimulated C cells in sheep with hypercalcaemia.
在通过每日给予维生素D3（200万国际单位）诱导产生高钙血症的绵羊中，发现C细胞增生和肥大。用维生素D3治疗10、20或30天后，血清钙值分别升至每分升10.28、11.86和10.44毫克，而正常浓度约为每分升9毫克。降钙素、嗜铬粒蛋白A和降钙素基因相关肽（CGRP）的免疫组化反应减弱，而在C细胞中观察到强烈的神经元特异性烯醇化酶（NSE）免疫反应性。用抗降钙素、抗嗜铬粒蛋白A、抗CGRP和抗NSE抗血清进行免疫组化染色，有助于证明高钙血症绵羊中受刺激的C细胞的功能状态。
