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在中性滤膜洗脱技术中，该滤膜并非作为DNA大小的鉴别器。

The filter does not act as a DNA size discriminator in the neutral filter elution technique.

作者信息

Nygren J, Ahnström G

机构信息

Stockholm University, Arrhenius Laboratories for Natural Sciences, Department of Radiobiology, Sweden.

出版信息

Int J Radiat Biol. 1992 Oct;62(4):397-9. doi: 10.1080/09553009214552271.

DOI:10.1080/09553009214552271
PMID:1357052
Abstract

Various types of filters were used with the neutral filter elution technique. No significant differences in elution rate or curve shapes were found. Therefore we assume that the release of DNA fragments from the DNA gel formed by lysis of cells is the rate-determining event in elution and not the rate of migration through the filter. We suggest that the filter only serves as a support for the DNA gel.

摘要

在中性滤膜洗脱技术中使用了各种类型的滤膜。未发现洗脱率或曲线形状有显著差异。因此，我们认为细胞裂解形成的DNA凝胶中DNA片段的释放是洗脱过程中的限速事件，而不是通过滤膜的迁移速率。我们认为滤膜仅作为DNA凝胶的支撑物。

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