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非变性DNA滤膜洗脱技术中裂解液成分及对电离辐射的非线性剂量反应

Lysis solution composition and non-linear dose-response to ionizing radiation in the non-denaturing DNA filter elution technique.

作者信息

Radford I R

机构信息

Molecular Science Group, Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia.

出版信息

Int J Radiat Biol. 1990 Mar;57(3):479-83. doi: 10.1080/09553009014552621.

DOI:10.1080/09553009014552621
PMID:1968941
Abstract

The suggestion by Okayasu and Iliakis (1989) that the non-linear dose-response curve, obtained with the non-denaturing filter elution technique for mammalian cells exposed to low-LET radiation, is the result of a technical artefact, was not confirmed.

摘要

冈安和伊利亚基斯(1989年)提出,用非变性滤膜洗脱技术对暴露于低传能线密度辐射的哺乳动物细胞所获得的非线性剂量反应曲线是技术假象的结果,但这一观点未得到证实。

相似文献

1
Lysis solution composition and non-linear dose-response to ionizing radiation in the non-denaturing DNA filter elution technique.非变性DNA滤膜洗脱技术中裂解液成分及对电离辐射的非线性剂量反应
Int J Radiat Biol. 1990 Mar;57(3):479-83. doi: 10.1080/09553009014552621.
2
The shape of DNA elution dose-response curves under non-denaturing conditions: the contribution of the degree of chromatin condensation.
Int J Radiat Biol. 1992 Apr;61(4):455-63. doi: 10.1080/09553009214551211.
3
Non-linear dose-effect curve for DNA double-strand breaks by low LET radiation: the effect of eluting buffer composition on the measurement of breaks by the filter elution technique.
Int J Radiat Biol. 1989 Dec;56(6):943-50. doi: 10.1080/09553008914552401.
4
Detection of ionizing radiation-induced DNA double-strand breaks by filter elution is affected by nuclear chromatin structure.通过滤膜洗脱法检测电离辐射诱导的DNA双链断裂会受到核染色质结构的影响。
Radiat Res. 1990 Dec;124(3):309-16.
5
The filter does not act as a DNA size discriminator in the neutral filter elution technique.在中性滤膜洗脱技术中,该滤膜并非作为DNA大小的鉴别器。
Int J Radiat Biol. 1992 Oct;62(4):397-9. doi: 10.1080/09553009214552271.
6
Linear DNA elution dose response curves obtained in CHO cells with non-unwinding filter elution after appropriate selection of the lysis conditions.在适当选择裂解条件后,通过非解旋滤膜洗脱在CHO细胞中获得的线性DNA洗脱剂量反应曲线。
Int J Radiat Biol. 1989 Apr;55(4):569-81. doi: 10.1080/09553008914550621.
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The dose-response for low-LET radiation-induced DNA double-strand breakage: methods of measurement and implications for radiation action models.低传能线密度辐射诱导DNA双链断裂的剂量反应:测量方法及其对辐射作用模型的意义
Int J Radiat Biol. 1988 Jul;54(1):1-11. doi: 10.1080/09553008814551451.
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Role of protein thiols in intrinsic radiation protection of DNA and cells.蛋白质硫醇在DNA和细胞的内在辐射防护中的作用。
Mutat Res. 1993 May;299(3-4):261-9. doi: 10.1016/0165-1218(93)90102-j.
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Size distribution of DNA molecules recovered from non-denaturing filter elution.
Int J Radiat Biol. 1991 Apr;59(4):919-26. doi: 10.1080/09553009114550811.
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Neutral filter elution detects differences in chromatin organization which can influence cellular radiosensitivity.中性滤膜洗脱法可检测染色质结构的差异,而这种差异会影响细胞的放射敏感性。
Radiat Res. 1992 Nov;132(2):242-7.

引用本文的文献

1
Induction, repair and biological relevance of radiation-induced DNA lesions in eukaryotic cells.真核细胞中辐射诱导的DNA损伤的诱导、修复及生物学相关性
Radiat Environ Biophys. 1990;29(4):273-92. doi: 10.1007/BF01210408.