Beckman G, Beckman L, Sikström C, Millán J L
Department of Medical Genetics, University of Umeå, Sweden.
Am J Hum Genet. 1992 Nov;51(5):1066-70.
The use of human placental alkaline phosphatase (PLAP) cDNA as a probe allows the detection and identification of restriction DNA fragments derived from three homologous genes, i.e., intestinal alkaline phosphatase (AP), germ-cell AP (GCAP), and PLAP. In previous RFLP studies we have reported linkage disequilibria between an RsaI and two PstI (a and b) polymorphic restriction sites and electrophoretic types of PLAP. In this report we present evidence that, in spite of the strong correlation with PLAP types, PstI(b) is an RFLP of GCAP. The data indicate close linkage between the PLAP and GCAP loci.
使用人胎盘碱性磷酸酶（PLAP）cDNA作为探针，可以检测和鉴定来自三个同源基因的限制性DNA片段，即肠碱性磷酸酶（AP）、生殖细胞AP（GCAP）和PLAP。在先前的限制性片段长度多态性（RFLP）研究中，我们报道了RsaI与两个PstI（a和b）多态性限制性位点之间的连锁不平衡以及PLAP的电泳类型。在本报告中，我们提供证据表明，尽管与PLAP类型有很强的相关性，但PstI(b)是GCAP的RFLP。数据表明PLAP和GCAP基因座之间存在紧密连锁。
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