Nørremølle A, Sørensen S A, Arctander P
Institute of Medical Genetics, University of Copenhagen, Denmark.
Clin Genet. 1992 Oct;42(4):210-1. doi: 10.1111/j.1399-0004.1992.tb03240.x.
The nucleotide sequence of the probe BS674E-D defining the genetic locus D4S95 was assessed, and used for designing PCR-primers amplifying a 175 bp fragment containing a polymorphic MboI site. With the described conditions it is now possible to use PCR followed by digestion for rapid determination of genotype in this marker system.
对定义基因座D4S95的探针BS674E-D的核苷酸序列进行了评估，并用于设计扩增包含多态性MboI位点的175bp片段的PCR引物。在所述条件下，现在可以使用PCR随后进行酶切来快速确定该标记系统中的基因型。
