Tachdjian G, Benabdennebi M, Guidal C, Sayada C, Lapouméroulie C, Elion J
Laboratoire de Biochimie Génétique, Hôpital Robert Debré, Paris, France.
Hum Genet. 1992 Sep-Oct;90(1-2):23-6. doi: 10.1007/BF00210740.
The GC-->TT polymorphism recently described at positions -1106 and -1105 in the 5' flanking region of the G gamma globin gene for the Bantu beta S haplotype was analysed by denaturing gradient gel electrophoresis. We studied 108 beta S chromosome and 122 beta A chromosomes. The TT sequence was found as follows: in all of 80 chromosomes bearing the Bantu beta S haplotype with the 6-bp deletion -400 nt from the G gamma gene in 3 out of 5 Bantu beta S chromosomes without the deletion, in 1 out of 122 beta A chromosomes from different ethnic origins but in none of 23 beta S chromosomes bearing the Senegal, Benin or Cameroon haplotypes. These results confirm the heterogeneity of the Bantu beta S haplotype and allow a tentative evolutionary sequence for the different alleles at this locus to be presented.
采用变性梯度凝胶电泳法分析了最近报道的班图βS单倍型Gγ珠蛋白基因5'侧翼区-1106和-1105位点的GC→TT多态性。我们研究了108条βS染色体和122条βA染色体。发现TT序列如下：在所有80条携带班图βS单倍型且Gγ基因缺失6bp（距Gγ基因-400nt）的染色体中，在5条无缺失的班图βS染色体中有3条，在122条来自不同种族的βA染色体中有1条，但在23条携带塞内加尔、贝宁或喀麦隆单倍型的βS染色体中均未发现。这些结果证实了班图βS单倍型的异质性，并给出了该位点不同等位基因的初步进化序列。
