• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.

作者信息

Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H

出版信息

Cold Spring Harb Symp Quant Biol. 1986;51 Pt 1:263-73. doi: 10.1101/sqb.1986.051.01.032.

DOI:10.1101/sqb.1986.051.01.032
PMID:3472723
Abstract
摘要

相似文献

1
Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.DNA的体外特异性酶促扩增:聚合酶链反应
Cold Spring Harb Symp Quant Biol. 1986;51 Pt 1:263-73. doi: 10.1101/sqb.1986.051.01.032.
2
The unusual origin of the polymerase chain reaction.聚合酶链反应的非凡起源。
Sci Am. 1990 Apr;262(4):56-61, 64-5. doi: 10.1038/scientificamerican0490-56.
3
Use of polymerase chain reaction catalyzed by Taq DNA polymerase for site-specific mutagenesis.使用Taq DNA聚合酶催化的聚合酶链反应进行位点特异性诱变。
Gene. 1989 Mar 15;76(1):161-6. doi: 10.1016/0378-1119(89)90018-8.
4
PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes.特定等位基因的聚合酶链反应扩增(PASA)是一种快速检测已知单碱基变化的通用方法。
Biotechniques. 1992 Jan;12(1):82-7.
5
DNA damage promotes jumping between templates during enzymatic amplification.DNA损伤会在酶促扩增过程中促进模板间的跳跃。
J Biol Chem. 1990 Mar 15;265(8):4718-21.
6
Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.克隆至M13载体的DNA片段的寡核苷酸定向诱变
Methods Enzymol. 1983;100:468-500. doi: 10.1016/0076-6879(83)00074-9.
7
Salt-dependent performance variation of DNA polymerases in co-amplification PCR.共扩增PCR中DNA聚合酶的盐依赖性性能变化
Biotechniques. 1996 Jul;21(1):28-30. doi: 10.2144/96211bm04.
8
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
9
[Simple methods of enzymatic amplification of DNA for clinical practice].[用于临床实践的DNA酶促扩增简单方法]
Mol Gen Mikrobiol Virusol. 1991 Aug(8):6-8.
10
GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects.通过反向PCR进行富含GC模板的扩增。DNA聚合酶和溶剂效应。
Methods Mol Biol. 2002;192:75-80. doi: 10.1385/1-59259-177-9:075.

引用本文的文献

1
Recent advances in techniques used in the diagnosis of lumpy skin disease: a review.牛结节性皮肤病诊断技术的最新进展:综述
J Vet Diagn Invest. 2025 Sep 9:10406387251373081. doi: 10.1177/10406387251373081.
2
Separation of Transcription-Derived Duplicates From PCR Duplicates to Enhance Sequence Data Utilization.从PCR重复序列中分离转录衍生重复序列以提高序列数据利用率。
Bioinform Biol Insights. 2025 Aug 26;19:11779322251365042. doi: 10.1177/11779322251365042. eCollection 2025.
3
A Sliding Microfluidic Chip-Integrated Colorimetric Biosensor Using MnO Nanoflowers for Rapid Detection.
一种基于MnO纳米花的集成滑动微流控芯片比色生物传感器用于快速检测
Micromachines (Basel). 2025 Jul 31;16(8):904. doi: 10.3390/mi16080904.
4
Integrated horizontal convective PCR system for clinical diagnostics.用于临床诊断的集成式水平对流PCR系统。
Sci Adv. 2025 Aug 8;11(32):eadx8434. doi: 10.1126/sciadv.adx8434. Epub 2025 Aug 6.
5
The Power of Old Hats: Rediscovering Inosine-EpPCR to Create Starting Libraries for Whole-Cell-SELEX.旧方法的力量:重新发现肌苷易错PCR以创建用于全细胞SELEX的起始文库。
Biosensors (Basel). 2025 Jul 12;15(7):448. doi: 10.3390/bios15070448.
6
Digital PCR: from early developments to its future application in clinics.数字PCR:从早期发展到其在临床中的未来应用
Lab Chip. 2025 Jul 21. doi: 10.1039/d5lc00055f.
7
Biogenic Zinc nanoparticles: green approach to synthesis, characterization, and antimicrobial applications.生物源锌纳米颗粒:绿色合成方法、表征及抗菌应用
Microb Cell Fact. 2025 Jul 18;24(1):168. doi: 10.1186/s12934-025-02788-9.
8
Comprehensive performance evaluation of a high-throughput automated system for pathogen nucleic acid detection in clinical settings.临床环境中病原体核酸检测高通量自动化系统的综合性能评估
Front Microbiol. 2025 Jun 16;16:1609142. doi: 10.3389/fmicb.2025.1609142. eCollection 2025.
9
qByte: An open-source isothermal fluorimeter for democratizing analysis of nucleic acids, proteins and cells.qByte:一款用于普及核酸、蛋白质和细胞分析的开源等温荧光计。
PLoS Biol. 2025 May 28;23(5):e3003199. doi: 10.1371/journal.pbio.3003199. eCollection 2025 May.
10
Development of Visual Loop-Mediated Isothermal Amplification Assays for Foodborne Hepatitis A Virus.食源性甲型肝炎病毒可视化环介导等温扩增检测方法的开发
Foods. 2025 Mar 10;14(6):934. doi: 10.3390/foods14060934.