使用放线菌素 D 抗性小鼠骨髓瘤细胞系构建针对α-内啡肽/辣根过氧化物酶的四瘤细胞。
Construction of a quadroma to alpha-endorphin/horseradish peroxidase using an actinomycin D-resistant mouse myeloma cell line.
作者信息
Kizim E A, Dergunova N N, Vostrikov V M, Dmitriev A D
机构信息
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow.
出版信息
Immunol Lett. 1992 Aug;33(3):217-22. doi: 10.1016/0165-2478(92)90064-u.
A hybrid hybridoma (quadroma), secreting antibodies with double specificity to alpha-endorphin (alpha-EP) and horseradish peroxidase (HRP), has been produced. The bispecific antibodies constituted about 28-29% of all immunologically active IgG, produced by quadroma. The quadroma was isolated by fusion of two mouse hybridomas (anti-HRP and anti-alpha-EP) with distinct phenotypes: double mutant AMDR/HAT(S), and wild type (AMDS/HATR). A novel strategy for the construction of a double-mutant was applied, based on the use of an actinomycin D-resistant (AMDR) mouse myeloma for initiation of one of the parental hybridomas.
已制备出一种分泌对α-内啡肽（α-EP）和辣根过氧化物酶（HRP）具有双特异性抗体的杂交杂交瘤（四瘤）。双特异性抗体约占四瘤产生的所有免疫活性IgG的28%-29%。通过将两种具有不同表型的小鼠杂交瘤（抗HRP和抗α-EP）融合来分离四瘤：双突变体AMDR/HAT(S)和野生型（AMDS/HATR）。基于使用抗放线菌素D（AMDR）小鼠骨髓瘤来启动亲本杂交瘤之一，应用了一种构建双突变体的新策略。
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