Chervonsky A V, Faerman A I, Evdonina L V, Jazova A K, Kazarov A R, Gussev A I
Laboratory of Immunochemistry, Cancer Research Center, USSR Academy of Medical Sciences, Moscow.
Mol Immunol. 1988 Sep;25(9):913-5. doi: 10.1016/0161-5890(88)90129-0.
A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.
已提出一种代谢选择系统，用于基于在一个亲本细胞系中同时引入两个性状——一个隐性性状（对8-氮杂鸟嘌呤的抗性）和一个显性性状（多药耐药性）来选择杂交杂交瘤（四元瘤）。在两种选择剂（氨基蝶呤和放线菌素D）存在的情况下选择四元瘤。使用这种方法，我们制备了分泌针对辣根过氧化物酶和人甲胎蛋白的双特异性单克隆抗体的四元瘤。
