Kasai K, Sato Y, Kuwao S, Kawakubo Y, Inoue H, Kameya T
Department of Pathology, Kitasato University School of Medicine, Kanagawa, Japan.
J Cancer Res Clin Oncol. 1992;119(2):87-90. doi: 10.1007/BF01209661.
The expression of sialosylated Lewis chi (SLEX), a ligand for endothelial leukocyte adhesion molecule 1 in malignant lymphomas, was immunohistochemically examined, using the monoclonal antibody, CSLEX1, which specifically reacts with SLEX. It was expressed in 6 out of 64 non-Hodgkin's lymphomas, which consisted of 1 nasal large-cell lymphoma and 5 of 8 (62%) Ki-1-positive anaplastic large-cell lymphomas (ALCL). One nasal lymphoma positive for SLEX co-expressed a T cell marker, cluster of differentiation (CD) 5, and natural killer (NK) cell markers such as CD56 and CD16, indicating that SLEX+ nasal lymphoma cells are possibly malignant counterparts of SLEX+ NK cells. SLEX did not react with 30 B cell lymphomas or most Hodgkin's disease lymphomas, though it did with one lymphocyte predominance type. Although SLEX+ ALCL exhibit T cell markers in some cases, some ALCL expressing SLEX may represent histiocytic differentiation of the neoplastic cells. The lymphoma cells of ALCL were preferentially positive for SLEX, in contrast to Hodgkin's disease cells, and thus CSLEX1 in conjunction with CD30 and CD15 should be of use for analyzing and making differential diagnoses of routine paraffin-embedded sections of ALCL.
使用与唾液酸化路易斯χ（SLEX）特异性反应的单克隆抗体CSLEX1，通过免疫组织化学方法检测了恶性淋巴瘤中内皮白细胞黏附分子1的配体SLEX的表达情况。在64例非霍奇金淋巴瘤中，有6例表达SLEX，其中包括1例鼻腔大细胞淋巴瘤以及8例（62%）Ki-1阳性间变性大细胞淋巴瘤（ALCL）中的5例。1例SLEX阳性的鼻腔淋巴瘤同时表达T细胞标志物分化簇（CD）5以及自然杀伤（NK）细胞标志物如CD56和CD16，这表明SLEX阳性的鼻腔淋巴瘤细胞可能是SLEX阳性NK细胞的恶性对应物。SLEX与30例B细胞淋巴瘤或大多数霍奇金病淋巴瘤不发生反应，不过与1例淋巴细胞为主型淋巴瘤有反应。尽管有些SLEX阳性的ALCL在某些情况下表现出T细胞标志物，但一些表达SLEX的ALCL可能代表肿瘤细胞的组织细胞分化。与霍奇金病细胞相比，ALCL的淋巴瘤细胞优先表达SLEX，因此CSLEX1与CD30和CD15联合应用，应有助于分析和鉴别诊断ALCL的常规石蜡包埋切片。
