色氨酸抑制注射了大鼠脑信使核糖核酸的非洲爪蟾卵母细胞对[3H]谷氨酸的摄取。

Tryptophan inhibits the [3H]glutamate uptake into Xenopus oocytes injected with rat brain mRNA.

作者信息

Tohda M, Urushihara H, Nomura Y

机构信息

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

出版信息

Jpn J Pharmacol. 1992 Aug;59(4):477-9. doi: 10.1254/jjp.59.477.

DOI:10.1254/jjp.59.477

PMID:1359181

Abstract

We characterized the glutamate (Glu) uptake in Xenopus oocytes injected with rat brain mRNA. The Glu uptake into oocytes was higher in mRNA-injected oocytes than in vehicle-injected ones. Na+ omission or addition of tryptophan inhibited the uptake in mRNA-injected oocytes, although it did not affect that in vehicle-injected oocytes. These results suggest that Glu transporters with a tryptophan sensitivity different from that of Glu transporters in native oocytes are expressed after injection of rat brain mRNA.

摘要

我们对注射了大鼠脑mRNA的非洲爪蟾卵母细胞中的谷氨酸（Glu）摄取进行了表征。注射mRNA的卵母细胞对Glu的摄取高于注射溶剂的卵母细胞。去除Na⁺或添加色氨酸会抑制注射mRNA的卵母细胞中的摄取，尽管这对注射溶剂的卵母细胞中的摄取没有影响。这些结果表明，注射大鼠脑mRNA后表达了色氨酸敏感性与天然卵母细胞中的Glu转运体不同的Glu转运体。