大鼠脑钠非依赖性异亮氨酸转运活性在非洲爪蟾卵母细胞中的表达。
Expression of Na+-independent isoleucine transport activity from rat brain in Xenopus laevis oocytes.
作者信息
Bröer S, Bröer A, Hamprecht B
机构信息
Physiologisch-Chemisches Institut der Universität, Tübingen, Germany.
出版信息
Biochim Biophys Acta. 1994 Jun 1;1192(1):95-100. doi: 10.1016/0005-2736(94)90147-3.
Poly(A)+ RNA from C6-BU-1 rat glioma cells and rat astroglial cells induced isoleucine transport activity when injected into Xenopus laevis oocytes. The Na+-independent component of isoleucine transport was inhibited by leucine, phenylalanine and 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) but neither by methylaminoisobutyric acid (MeAIB) nor lysine. A Km value of approx. 100 microM was determined for the Na+-independent transport of isoleucine. These data are in accordance with expression of a system L like transporter. By injection of size fractionated poly(A)+ RNA a length of approx. 1.9 kb was determined for the pertinent mRNA.
将来自C6 - BU - 1大鼠胶质瘤细胞和大鼠星形胶质细胞的聚腺苷酸加尾（Poly(A)+）RNA注射到非洲爪蟾卵母细胞中时，可诱导异亮氨酸转运活性。异亮氨酸转运的非钠离子依赖成分受到亮氨酸、苯丙氨酸和2 - 氨基双环[2,2,1]庚烷 - 2 - 羧酸（BCH）的抑制，但不受甲基氨基异丁酸（MeAIB）和赖氨酸的抑制。测定异亮氨酸非钠离子依赖转运的米氏常数（Km值）约为100微摩尔。这些数据与类L系统转运蛋白的表达一致。通过注射大小分级的聚腺苷酸加尾（Poly(A)+）RNA，确定相关信使核糖核酸（mRNA）的长度约为1.9千碱基对。
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