Roll J T, Haak-Frendscho M, Brown J F, Czuprynski C J
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
Microb Pathog. 1992 Jul;13(1):25-35. doi: 10.1016/0882-4010(92)90029-n.
In this study, we determined whether spleen cells from Listeria monocytogenes-immunized mice were cytolytic for Listeria-infected macrophages. Spleen cells freshly obtained from immunized donors were unable to lyse Listeria-infected macrophages unless they were first stimulated in vitro for 2-3 days with Concanavalin A (ConA) or L. monocytogenes. Spleen cells from non-immunized mice developed cytolytic activity after incubation with ConA, but not with L. monocytogenes. Cytolytic spleen cells demonstrated an equivalent ability to lyse uninfected and Listeria-infected thioglycollate elicited peritoneal macrophages. Maximal cytolysis required co-incubation of effector and target cells for 18-20 h. Spleen cell culture supernatants did not lyse macrophages, suggesting that cytolysis required direct contact. Preincubation of immune spleen cells with ConA decreased their ability to transfer anti-listeria resistance in the spleens, but not the livers of recipient mice. Depletion of CD4+ or CD8+ cells did not significantly reduce the ability of ConA-incubated Listeria-immune spleen cells to transfer resistance. Despite being cytolytic for Listeria-immune infected macrophages, ConA-stimulated non-immune spleen cells did not transfer anti-listeria resistance. These results indicate that cytolytic cells can be generated by short-term incubation of spleen cells with antigen or mitogen. The dissociation between in vitro cytolytic activity and ability to transfer protection, however, suggests that the two biological activities are not inextricably linked.
在本研究中，我们确定了来自单核细胞增生李斯特菌免疫小鼠的脾细胞是否对感染李斯特菌的巨噬细胞具有细胞溶解作用。刚从免疫供体获得的脾细胞不能裂解感染李斯特菌的巨噬细胞，除非它们首先在体外用刀豆蛋白A（ConA）或单核细胞增生李斯特菌刺激2 - 3天。未免疫小鼠的脾细胞在与ConA孵育后产生细胞溶解活性，但与单核细胞增生李斯特菌孵育则不产生。具有细胞溶解作用的脾细胞对未感染和感染李斯特菌的巯基乙酸诱导的腹腔巨噬细胞具有同等的裂解能力。最大细胞溶解作用需要效应细胞和靶细胞共同孵育18 - 20小时。脾细胞培养上清液不能裂解巨噬细胞，这表明细胞溶解需要直接接触。用ConA预孵育免疫脾细胞会降低它们在受体小鼠脾脏而非肝脏中传递抗李斯特菌抗性的能力。去除CD4 +或CD8 +细胞并没有显著降低经ConA孵育的李斯特菌免疫脾细胞传递抗性的能力。尽管ConA刺激的非免疫脾细胞对感染李斯特菌的免疫巨噬细胞具有细胞溶解作用，但它们并不能传递抗李斯特菌抗性。这些结果表明，脾细胞与抗原或有丝分裂原短期孵育可产生细胞溶解细胞。然而，体外细胞溶解活性与传递保护能力之间的分离表明，这两种生物学活性并非紧密相连。
