Schünzel G, Wolf G, Pomrenke U, Pomrenke C, Schmidt W
Institute of Biology, Medical Academy of Magdeburg, Germany.
Neuroscience. 1992 Jul;49(2):365-71. doi: 10.1016/0306-4522(92)90102-8.
Male Wistar rats administered repetitively with pentylenetetrazol developed a dose-dependent enhancement of seizure behaviour referred to as pentylenetetrazol kindling. After a daily dose of 40 mg pentylenetetrazol/kg or physiological saline (control rats) injected intraperitoneally for a period of two weeks, hippocampal tissue was studied autoradiographically for high-affinity uptake of [3H]glutamate and, by activity staining, for aspartate aminotransferase and glutamate dehydrogenase. Most prominent changes were found in neuropil areas known to be endowed with glutamatergic structures. The uptake capacity of glutamate decreased by 48% (maximum rate), whilst activities of aspartate aminotransferase and glutamate dehydrogenase elevated to 140 and 130%, respectively. Cytochrome c oxidase activity was found to be unaffected. The findings indicate an important role of factors of the glutamate metabolism in the kindling process with respect to the production, utilization, and availability of transmitter glutamate.
反复给予戊四氮的雄性Wistar大鼠出现了剂量依赖性的癫痫发作行为增强，称为戊四氮点燃。在每天腹腔注射40mg戊四氮/kg或生理盐水（对照大鼠），持续两周后，对海马组织进行放射自显影研究，以检测[3H]谷氨酸的高亲和力摄取，并通过活性染色检测天冬氨酸转氨酶和谷氨酸脱氢酶。在已知具有谷氨酸能结构的神经毡区域发现了最显著的变化。谷氨酸的摄取能力下降了48%（最大速率），而天冬氨酸转氨酶和谷氨酸脱氢酶的活性分别升高到140%和130%。发现细胞色素c氧化酶活性未受影响。这些发现表明谷氨酸代谢因子在点燃过程中对于递质谷氨酸的产生、利用和可用性具有重要作用。
