Vuorinen P
Department of Biomedical Sciences, University of Tampere, Finland.
Pharmacol Toxicol. 1992 Jun;70(6 Pt 1):463-7. doi: 10.1111/j.1600-0773.1992.tb00509.x.
The effects of exogenous guanosine 5'-triphosphate (GTP), guanosine 5'-(gamma-thio)triphosphate (GTP gamma S), cysteine and Trolox C, a water soluble vitamin E analogue, were studied on basal and nitrovasodilator-induced cyclic GMP formation in isolated human lymphocytes. Incubation of lymphocytes in the presence of GTP (0.1 mM) and GTP gamma S (0.1 mM) increased cyclic GMP more than twofold. SIN-1 and sodium nitroprusside dose-dependently increased cyclic GMP, but nitroglycerin and sodium nitrite were ineffective. GTP and GTP gamma S potentiated SIN-1 and sodium nitroprusside-induced cyclic GMP formation. In the presence of GTP and GTP gamma S, nitroglycerin, but not sodium nitrite, was able to increase lymphocyte cyclic GMP. Cysteine (1 mM) enhanced cyclic GMP formation induced by sodium nitroprusside and nitroglycerin. Trolox C (0.1 mM) potentiated SIN-1-induced cyclic GMP formation. These results indicate that exogenous GTP and GTP gamma S enhance guanylate cyclase stimulation by spontaneous nitric oxide releasers and nitroglycerin in lymphocytes. Cysteine, a redox-compound and Trolox C, an antioxidant, have different effects on guanylate cyclase activation by nitric oxide releasers, SIN-1 and sodium nitroprusside.
研究了外源性鸟苷 5'-三磷酸（GTP）、鸟苷 5'-（γ-硫代）三磷酸（GTPγS）、半胱氨酸和水溶性维生素 E 类似物 Trolox C 对离体人淋巴细胞中基础和硝基血管扩张剂诱导的环鸟苷酸（cGMP）形成的影响。在 GTP（0.1 mM）和 GTPγS（0.1 mM）存在下孵育人淋巴细胞，可使 cGMP 增加两倍以上。SIN-1 和硝普钠剂量依赖性地增加 cGMP，但硝酸甘油和亚硝酸钠无效。GTP 和 GTPγS 增强了 SIN-1 和硝普钠诱导的 cGMP 形成。在 GTP 和 GTPγS 存在下，硝酸甘油而非亚硝酸钠能够增加淋巴细胞 cGMP。半胱氨酸（1 mM）增强了硝普钠和硝酸甘油诱导的 cGMP 形成。Trolox C（0.1 mM）增强了 SIN-1 诱导的 cGMP 形成。这些结果表明，外源性 GTP 和 GTPγS 增强了淋巴细胞中自发性一氧化氮释放剂和硝酸甘油对鸟苷酸环化酶的刺激作用。半胱氨酸（一种氧化还原化合物）和 Trolox C（一种抗氧化剂）对一氧化氮释放剂、SIN-1 和硝普钠激活鸟苷酸环化酶有不同影响。
