Riutta A, Alanko J, Mucha I, Vapaatalo H
Department of Biomedical Sciences, University of Tampere, Finland.
Eur J Pharmacol. 1994 Sep 12;262(3):217-22. doi: 10.1016/0014-2999(94)90735-8.
The effects of Trolox C (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a vitamin E analogue, (60-900 microM) and SIN-1 (3-morpholino sydnonimine), a nitric oxide donor, (30-3000 microM) on arachidonic acid metabolism and on cyclic GMP formation in calcium ionophore A23187 (calcimycin)-stimulated human polymorphonuclear leukocytes were investigated. Trolox C elicited a dose dependent decrease in leukotriene B4 levels and increase in prostaglandin E2 levels but did not affect cyclic GMP levels. SIN-1 dose dependently inhibited leukotriene B4 and stimulated prostaglandin E2 and cyclic GMP formation. Dibutyryl cyclic GMP did not affect the formation of leukotriene B4 and prostaglandin E2. Trolox C (180 microM), which itself had no effect on cyclic GMP levels, enhanced the effect of SIN-1 (100 microM) on cyclic GMP levels more than 5-fold. The effects of SIN-1 on arachidonic acid metabolism seem to be independent of cyclic GMP and are probably due to nitric oxide. In this experimental model both Trolox C and SIN-1 have similar actions on the prostaglandin/leukotriene ratio, and Trolox C potentiates the SIN-1-induced increase in cyclic GMP levels.
研究了维生素E类似物Trolox C（6-羟基-2,5,7,8-四甲基色满-2-羧酸）（60 - 900微摩尔）和一氧化氮供体SIN-1（3-吗啉代西多硝酯）（30 - 3000微摩尔）对钙离子载体A23187（钙霉素）刺激的人多形核白细胞中花生四烯酸代谢和环鸟苷酸形成的影响。Trolox C引起白三烯B4水平呈剂量依赖性下降，前列腺素E2水平升高，但不影响环鸟苷酸水平。SIN-1剂量依赖性抑制白三烯B4并刺激前列腺素E2和环鸟苷酸的形成。二丁酰环鸟苷酸不影响白三烯B4和前列腺素E2的形成。本身对环鸟苷酸水平无影响的Trolox C（180微摩尔）使SIN-1（100微摩尔）对环鸟苷酸水平的作用增强了5倍以上。SIN-1对花生四烯酸代谢的影响似乎与环鸟苷酸无关，可能是由于一氧化氮所致。在该实验模型中，Trolox C和SIN-1对前列腺素/白三烯比值具有相似的作用，且Trolox C可增强SIN-1诱导的环鸟苷酸水平升高。
