Miura K, Baba S, Shirasawa H, Ju S T, Cohen A S, Shirahama T
Department of Pathology, Hamamatsu University School of Medicine, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1992;62(4):245-50. doi: 10.1007/BF02899688.
Although resident peritoneal cells from amyloidotic mice (amyloidotic peritoneal cells) are capable of processing the precursor protein of secondary amyloidosis, serum amyloid A (SAA) to amyloid fibrils, the peritoneum is a rare site for amyloid deposition. This is considered to be due to a deficiency of SAA in the peritoneum. To increase the supply of SAA to the peritoneum, ascitic fluid containing about the same protein constituents as in the serum was induced in mice. Amyloidotic peritoneal cells were packed in a microchamber which was shielded with filter membranes, and cultured in ascitic fluid supplemented with additional inflammatory factors. On the 7th day, Congo red-positive structures which showed green birefringence under polarized light were found inside and occasionally outside the chamber. By anti-AA or -SAA immunostaining, amyloid deposits and the cell surfaces of macrophages were positive. Immunologic depletion of T- and B-lymphocytes from the amyloidotic peritoneal cells did not adversely effect the amyloid formation in microchambers. These results suggest that either ascitic fluid containing sufficient amounts of SAA, or peritoneal macrophages with a high amyloid enhancing factor (AEF) activity are indispensable for AA amyloid fibrillogenesis in the peritoneum.
尽管来自淀粉样变性小鼠的腹膜常驻细胞（淀粉样变性腹膜细胞）能够将继发性淀粉样变性的前体蛋白血清淀粉样蛋白A（SAA）加工成淀粉样纤维，但腹膜是淀粉样沉积的罕见部位。这被认为是由于腹膜中SAA缺乏所致。为了增加腹膜中SAA的供应，在小鼠中诱导产生了含有与血清中大致相同蛋白质成分的腹水。将淀粉样变性腹膜细胞装入用滤膜屏蔽的微室中，并在补充了额外炎症因子的腹水中培养。在第7天，在微室内外发现了在偏振光下呈现绿色双折射的刚果红阳性结构。通过抗AA或-SAA免疫染色，淀粉样沉积物和巨噬细胞的细胞表面呈阳性。从淀粉样变性腹膜细胞中免疫清除T淋巴细胞和B淋巴细胞对微室中的淀粉样形成没有不利影响。这些结果表明，含有足够量SAA的腹水或具有高淀粉样增强因子（AEF）活性的腹膜巨噬细胞对于腹膜中AA淀粉样纤维形成是必不可少的。
