Itze L, Vesselinovitch S D, Rao K V
Physiol Bohemoslov. 1976;25(4):289-93.
The present report is a continuation of our previous studies on the observations of macromolecule precursors incorporated into the liver of newborn (1-10 days), infant (11-21 days) and young adult (41-50 days) old mice C57BLfemale X C3Hmale Fl and 3-hour interval circadian rhythm in livers of 15 day-old mice. 3H thymidine, 3H uridine and 3H leucine were used for studying incorporation into the macromolecules. DNA, RNA and protein were prepared by a modified Kirby's procedure. The data obtained indicate fluctuation of DNA activity approximately at 5-day intervals with a decreasing tendency up to 50 days of age. In 15-day-old mice, the peak of synthetic DNA activity was observed at 9.00 a.m. and the lowest values were found the next day at 6.00 a.m. Our results should provide fundamental data from which it would be possible to speculate whether the hepatocarcinogenic effect of different compounds with or without a special affinity to induce hepatoma may be correlated to different macromolecular synthetic activity.
本报告是我们之前关于观察大分子前体掺入新生（1 - 10天）、婴儿期（11 - 21天）和年轻成年（41 - 50天）的C57BL雌性×C3H雄性F1代小鼠肝脏以及15日龄小鼠肝脏中3小时间隔昼夜节律研究的延续。使用³H胸腺嘧啶核苷、³H尿苷和³H亮氨酸来研究它们掺入大分子的情况。DNA、RNA和蛋白质通过改良的柯比方法制备。所获得的数据表明，DNA活性大约每隔5天波动一次，至50日龄时呈下降趋势。在15日龄小鼠中，合成DNA活性的峰值在上午9点观察到，次日凌晨6点发现最低值。我们的结果应能提供基础数据，据此有可能推测不同化合物（无论有无诱导肝癌的特殊亲和力）的致癌作用是否可能与不同的大分子合成活性相关。
