Itze L, Vesselinovitch S D, Rao K V
Physiol Bohemoslov. 1975;24(1):9-22.
Studies have been presented concerning the definition of bioassay systems, in terms of macromolecular kinetics as it pertains in advance to ENU carciogenic sensitivity. Male mice (C57BL female times C3H male) 6 weeks of age were partially hepatectomized and labelled with thymidine 3-H, uridine 3-H and leucine 3-H as precursors for DNA, RNA and protein synthesis respectively. Specific activity of all these three parameters of liver, thymus and kidney were followed for 5 days at regular intervals, and for 6 weeks at randomly chosen intervals. The goal of this study which we obtained, is a three-phased curve in liver for each macromolecule studied; RNA synthesis reached its first peak 18 hours, protein synthesis 24 hours, and DNA synthesis 30 hours after partial hepatectomy. Synthetic macromolecular turnover in other organs (thymus, lung, kidney, brain) were not affected in a similar way, inspite expecting some factors of a humoral nature. An accurate knowledge of the timing in macromolecular synthesis after partial hepatectomy in mice is an essential preliminary study for the development and definition of a model protocol for ENU--single dose having approximately 10-15 minutes half-life carcinogenic response.
已经有关于生物测定系统定义的研究，该定义依据与ENU致癌敏感性预先相关的大分子动力学。6周龄的雄性小鼠（C57BL雌性与C3H雄性杂交）接受部分肝切除，并分别用3-H胸腺嘧啶核苷、3-H尿苷和3-H亮氨酸作为DNA、RNA和蛋白质合成的前体进行标记。对肝脏、胸腺和肾脏这三个参数的比活性每隔一定时间跟踪5天，并在随机选择的时间间隔内跟踪6周。我们这项研究得到的目标是，在所研究的每个大分子的肝脏中呈现出一条三相曲线；部分肝切除后18小时RNA合成达到第一个峰值，24小时蛋白质合成达到第一个峰值，30小时DNA合成达到第一个峰值。尽管预期存在一些体液性质的因素，但其他器官（胸腺、肺、肾脏、脑）中的合成大分子周转并未受到类似影响。准确了解小鼠部分肝切除后大分子合成的时间，对于开发和定义ENU单剂量致癌反应模型方案（半衰期约为10 - 15分钟）而言是一项必不可少的初步研究。
