Samoĭlina N L, Udalov G A
Biull Eksp Biol Med. 1992 Jun;113(6):655-8.
The ability of transplantable hemopoietic stem cells (HSC) to maintain their pool was studied using successive bone marrow transplantations with quantitative evaluation of hemopoiesis restoring units (HRU) in each transfer. The number of injected HRU increased (3.6-48.6--fold) upon each transfer; however, the normal level could not be attained. The ability fo HRU for further multiplication was exhausted after five transfers. HRU lost totipotentiality after four transfers. The data obtained support the concertion of Kay (1965) that HSC department is a pool of heterogeneous cells, and the property of "stemness" is inversely related to the number of divisions of ancestral cells. Transplantation, being a proliferative stress for the dormant HSCs, thus lowers the stem potential of the whole pool. The experimental data suggest that while dividing stem cell does not have a choice to self-renew or to differentiate into maturing cells, but it really differentiates into HSCs of lower rank.
利用连续骨髓移植并对每次移植中的造血恢复单位（HRU）进行定量评估，研究了可移植造血干细胞（HSC）维持其库的能力。每次移植时，注入的HRU数量增加（3.6至48.6倍）；然而，无法达到正常水平。五次移植后，HRU进一步增殖的能力耗尽。四次移植后，HRU失去了全能性。所获得的数据支持Kay（1965年）的观点，即造血干细胞库是一组异质性细胞，“干性”特性与祖细胞的分裂次数呈负相关。移植作为休眠造血干细胞的增殖应激，从而降低了整个库的干细胞潜能。实验数据表明，虽然分裂的干细胞没有自我更新或分化为成熟细胞的选择，但它实际上分化为等级较低的造血干细胞。
