Funkenstein B, Tandler A, Cavari B
National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel.
Mol Cell Endocrinol. 1992 Sep;87(1-3):R7-9. doi: 10.1016/0303-7207(92)90249-6.
Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of beta-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.
