金头鲷（Sparus aurata）生长激素编码cDNA的克隆与测序

Cloning and sequencing of the gilthead seabream (Sparus aurata) growth hormone-encoding cDNA.

作者信息

Funkenstein B, Chen T T, Powers D A, Cavari B

机构信息

Israel Oceanographic and Limnological Research, Haifa.

出版信息

Gene. 1991 Jul 22;103(2):243-7. doi: 10.1016/0378-1119(91)90280-o.

DOI:10.1016/0378-1119(91)90280-o

PMID:1889749

Abstract

The cDNA clones encoding gilthead seabream (gsb) (Sparus aurata) growth hormone (GH) have been isolated from a cDNA library prepared from seabream pituitary gland poly(A)+ RNA. The cDNA library was screened using red seabream and rainbow trout GH cDNAs. The complete nucleotide (nt) sequence of gsbGH has been determined. The cDNA sequence codes for a polypeptide of 204 amino acids (aa), including a putative signal peptide of 17 aa. The 5'- and 3'-untranslated regions of the message are 55 and 236 nt long, respectively. The predicted aa sequence of gsbGH revealed 97% homology with red seabream GH, 95% with tuna GH, 85% with yellowtail GH, and 65% with rainbow trout GH.

摘要

已从由金头鲷垂体腺多聚腺苷酸加尾RNA制备的cDNA文库中分离出编码金头鲷（gsb）（Sparus aurata）生长激素（GH）的cDNA克隆。使用红鲷和虹鳟鱼GH cDNA对该cDNA文库进行筛选。已确定gsbGH的完整核苷酸（nt）序列。该cDNA序列编码一个由204个氨基酸（aa）组成的多肽，包括一个17个氨基酸的假定信号肽。该信使RNA的5'非翻译区和3'非翻译区分别长55和236 nt。预测的gsbGH氨基酸序列与红鲷GH有97%的同源性，与金枪鱼GH有95%的同源性，与黄尾GH有85%的同源性，与虹鳟鱼GH有65%的同源性。