Lehner T, Bergmeier L A, Panagiotidi C, Tao L, Brookes R, Klavinskis L S, Walker P, Walker J, Ward R G, Hussain L
Division of Immunology, United Medical School, Guy's Hospital, London, United Kingdom.
Science. 1992 Nov 20;258(5086):1365-9. doi: 10.1126/science.1360702.
Heterosexual transmission through the cervico-vaginal mucosa is the principal route of human immunodeficiency virus (HIV) infection in Africa and is increasing in the United States and Europe. Vaginal immunization with simian immunodeficiency virus (SIV) had not yet been studied in nonhuman primates. Immune responses in macaques were investigated by stimulation of the genital and gut-associated lymphoid tissue with a recombinant, particulate SIV antigen. Vaginal, followed by oral, administration of the vaccine elicited three types of immunity: (i) gag protein p27-specific, secretory immunoglobulin A (IgA) and immunoglobulin G (IgG) in the vaginal fluid, (ii) specific CD4+ T cell proliferation and helper function in B cell p27-specific IgA synthesis in the genital lymph nodes, and (iii) specific serum IgA and IgG, with CD4+ T cell proliferative and helper functions in the circulating blood.
通过宫颈-阴道黏膜进行的异性传播是非洲人类免疫缺陷病毒（HIV）感染的主要途径，在美国和欧洲这种传播方式也在增加。在非人类灵长类动物中尚未对用猿猴免疫缺陷病毒（SIV）进行阴道免疫进行研究。通过用重组颗粒状SIV抗原刺激生殖器和肠道相关淋巴组织来研究猕猴的免疫反应。经阴道给药，随后口服该疫苗可引发三种免疫类型：（i）阴道液中针对gag蛋白p27的分泌型免疫球蛋白A（IgA）和免疫球蛋白G（IgG），（ii）生殖器淋巴结中特异性CD4 + T细胞增殖以及在B细胞p27特异性IgA合成中的辅助功能，以及（iii）特异性血清IgA和IgG，同时在循环血液中具有CD4 + T细胞增殖和辅助功能。
