Ambrosi G, Nicolardi G, Rizzi A, Camosso M E, Benagiano V, Arena R, Fanelli F
Institute of Human Anatomy, University of Bari.
Boll Soc Ital Biol Sper. 1992 May;68(5):321-8.
Further immunocytochemical analysis of the neuroblasts with SRIF-like immunoreactivity (ir) was carried out on the chick embryo medulla and pons. 5 or 100 microns rombencephalon sections were obtained from 60 White Leghorn chick embryos at stages (E = Embryonic days) ranging from E4 1/2 to E18 and incubated with rabbit polyclonal antibodies against synthetic cyclic Somatostatin-14, according to PAP-DAB technique. In the medulla and pons the ir appeared as from E12. From E12 to E13 1/2-E14 the ir distribution gradually changed. From E14 to E18 numbers and spatial arrangement of the positive neuroblast groups did not show substantial changes; in these respects the ir distributional pattern proved to be markedly different from the one observed by the Authors in adult animals. Moreover, from E13 to E15 the positive neuroblast density appeared to be higher than that of positive neurons in adults. These results are consistent with a possible SRIF local regulative role.
对具有生长抑素释放抑制因子样免疫反应性（ir）的神经母细胞进行了进一步的免疫细胞化学分析，研究对象为鸡胚延髓和脑桥。从60只处于E4 1/2至E18阶段（E =胚胎日）的白来航鸡胚中获取5或100微米厚的后脑切片，并根据PAP - DAB技术，用抗合成环生长抑素 - 14的兔多克隆抗体进行孵育。在延髓和脑桥中，ir从E12开始出现。从E12到E13 1/2 - E14，ir分布逐渐变化。从E14到E18，阳性神经母细胞群的数量和空间排列没有明显变化；在这些方面，ir分布模式被证明与作者在成年动物中观察到的明显不同。此外，从E13到E15，阳性神经母细胞密度似乎高于成年动物中的阳性神经元密度。这些结果与生长抑素释放抑制因子可能的局部调节作用一致。
