海胆卵中的腺苷酸环化酶受D-1和D-2多巴胺受体的正向和负向调节。

Adenylate cyclase from sea urchin eggs is positively and negatively regulated by D-1 and D-2 dopamine receptors.

作者信息

Carginale V, Capasso A, Madonna L, Borrelli L, Parisi E

机构信息

C.N.R. Institute of Protein Biochemistry and Enzymology, Naples, Italy.

出版信息

Exp Cell Res. 1992 Dec;203(2):491-4. doi: 10.1016/0014-4827(92)90026-5.

DOI:10.1016/0014-4827(92)90026-5

PMID:1360904

Abstract

The adenylate cyclase present in membranes prepared from sea urchin eggs is sensitive to dopamine stimulation. The receptor sites coupled to sea urchin adenylate cyclase were characterized by means of specific agonists and antagonists. The D-1 dopamine agonist SKF-38393 was able to stimulate enzyme activity, while the two D-1 dopamine antagonists, SCH-23390 and SKF-83566, suppressed the stimulatory effect of dopamine. In addition, the D-2 dopamine agonists, PPHT and metergoline, brought about a dose-dependent inhibition of dopamine-stimulated adenylate cyclase activity. These data show that: (i) in sea urchin eggs adenylate cyclase is regulated by dopamine receptors; (ii) these receptors share characteristics with D-1 and D-2 dopamine receptors present in the mammalian brain.

摘要

从海胆卵制备的膜中存在的腺苷酸环化酶对多巴胺刺激敏感。通过特异性激动剂和拮抗剂对与海胆腺苷酸环化酶偶联的受体位点进行了表征。D-1多巴胺激动剂SKF-38393能够刺激酶活性，而两种D-1多巴胺拮抗剂SCH-23390和SKF-83566则抑制了多巴胺的刺激作用。此外，D-2多巴胺激动剂PPHT和麦角新碱对多巴胺刺激的腺苷酸环化酶活性产生了剂量依赖性抑制。这些数据表明：（i）在海胆卵中，腺苷酸环化酶受多巴胺受体调节；（ii）这些受体与哺乳动物脑中存在的D-1和D-2多巴胺受体具有共同特征。