普通章鱼神经系统中一种对多巴胺和章鱼胺敏感的腺苷酸环化酶。

A dopamine- and octopamine-sensitive adenylate cyclase in the nervous system of Octopus vulgaris.

Capasso A, Carginale V, Madonna L, Mancaniello D, Scudiero R, Paolo De Prisco P, De Petrocellis B, Parisi E

C.N.R. Institute of Protein Biochemistry and Enzymology, Napoli, Italy.

Comp Biochem Physiol B. 1991;100(4):805-8. doi: 10.1016/0305-0491(91)90294-n.

Abstract

Adenylate cyclase activity was assayed in the optic lobe of Octopus vulgaris. 2. Both octopamine and dopamine stimulate the octopus adenylate cyclase, apparently by competing with the same receptor site. 3. (+/-)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene-HBr (6,7-ADTN) and a number of phenylethanolamine derivatives stimulate the octopus adenylate cyclase activity. 4. The dopamine D-1 antagonists R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl- 2,3,4,5-tetrahydro-1H-3-benzazepine-HCl (SCH-23390) and (+/-)-7-bromo-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H- 3-benzazepine-HCl (SKF-83566) are unable to antagonize the effects of dopamine and octopamine, and similarly ineffective is the agonist (+/-)-1-phenyl-2,3,4,5-tetrahydro-1H-3- benzazepine-7,8-diol-HCl (SKF-38393). 5. No detectable binding of labelled SCH-23390 occurs on membrane preparations from octopus optic lobe.

摘要

测定了普通章鱼视叶中的腺苷酸环化酶活性。2. 章鱼胺和多巴胺均能刺激章鱼腺苷酸环化酶，显然是通过与同一受体位点竞争来实现的。3. （±）-2-氨基-6,7-二羟基-1,2,3,4-四氢萘溴化氢（6,7-ADTN）和一些苯乙醇胺衍生物能刺激章鱼腺苷酸环化酶活性。4. 多巴胺D-1拮抗剂R（+）-7-氯-8-羟基-3-甲基-1-苯基-2,3,4,5-四氢-1H-3-苯并氮杂卓盐酸盐（SCH-23390）和（±）-7-溴-8-羟基-3-甲基-1-苯基-2,3,4,5-四氢-1H-3-苯并氮杂卓盐酸盐（SKF-83566）无法拮抗多巴胺和章鱼胺的作用，同样无效的还有激动剂（±）-1-苯基-2,3,4,5-四氢-1H-3-苯并氮杂卓-7,8-二醇盐酸盐（SKF-38393）。5. 在章鱼视叶的膜制剂上未检测到标记的SCH-23390的结合。