FULTHORPE A J
Immunology. 1958 Oct;1(4):365-72.
Comparative quantitative estimations of tetanus toxoid by different methods have been made. The total combining power test is considered likely to give a true assessment of the combination of toxoid with antitoxin since it is based on the biological action of toxin. The haemagglutination inhibition test has been found to give good agreement with the test and it is cheap, sensitive and readily repeatable. Since the haemagglutination inhibition test can be performed with toxin or toxoid by the same method, it is possible to relate the quantitative estimation of toxoid to the L+ dose of a routine test toxin, and thus to a standard antitoxin, which is convenient. Considerable discrepancies have been found between results in the flocculation test and the total combining power test, particularly with toxoids denatured by heat and phenol, or modified by formalin. Discrepanicies between the flocculation and tests with many preparations have been related to the concentration of residual free formalin.
已采用不同方法对破伤风类毒素进行了比较定量评估。总结合力试验被认为可能会对类毒素与抗毒素的结合给出真实评估，因为它基于毒素的生物学作用。已发现血凝抑制试验与该试验结果吻合良好，且该试验成本低、灵敏度高且易于重复。由于血凝抑制试验可用相同方法对毒素或类毒素进行检测，因此有可能将类毒素的定量评估与常规试验毒素的L +剂量相关联，进而与标准抗毒素相关联，这很方便。已发现絮状试验结果与总结合力试验结果之间存在相当大的差异，尤其是对于经加热和苯酚变性或经福尔马林改性的类毒素。许多制剂在絮状试验和其他试验之间的差异与残留游离福尔马林的浓度有关。
