FULTHORPE A J
Immunology. 1962 Jan;5(1):30-45.
A fair degree of correlation has been found between the antitoxin content of sera from horses immunized with crude culture filtrates and the direct agglutinin titre of the sera when tested with sheep cells sensitized with diphtheria toxoid. Haemagglutination inhibition tests at the L level of test with the same sera showed some rather large discrepancies from the and further tests with special agglutinin inhibiting toxins suggested that specific antitoxin free from other accessory antibodies might be non-agglutinating, and therefore not titratable by haemagglutination inhibition. The phosphate-stable, pepsin-stable and trypsin-stable antigens isolated from culture filtrates of were found to contain extremely small quantities of specific toxoid, and cross titration of each of the three antigen preparations showed that there was very little contamination by other antigens within the group. Absorption of diphtheria antiserum with red cells sensitized with each of the three accessory antigens individually, showed that the antibodies were highly specific and distinct. Absorption of diphtheria antiserum with a mixture of red cells sensitized with the three different antigens removed all demonstrable accessory antibodies, and the absorbed serum would no longer agglutinate cells sensitized with complete diphtheria toxoid. The absorbed serum, however, retained a large proportion of its neutralizing capacity for diphtheria toxin, when titrated . Titration of each of the accessory antibodies in a number of horse sera by haemagglutination inhibition demonstrated a correlation between the values for the accessory antibodies to the phosphate-stable and pepsin-stable antigens, but no correlation with the values for the antibody to the trypsin-stable antigen, when compared with results of the flocculation test. The relative proportions of diphtheria toxin and of the phosphate-stable and pepsin-stable antigens remained constant at all stages of purification of a filtrate including several recrystallization procedures. However, the concentration of the trypsin-stable antigen declined steadily during the process. The relative proportions of toxin, phosphate-stable and pepsin-stable antigens in several crude culture filtrates were constant under reasonable conditions of storage and very varied where deterioration had occurred. These three antigens also maintained a constant ratio in growing culture, the trypsin-stable antigen did not.
在用粗培养滤液免疫的马血清中的抗毒素含量与用白喉类毒素致敏的绵羊细胞检测血清时的直接凝集素效价之间，已发现有一定程度的相关性。用相同血清在试验的L水平进行血凝抑制试验，结果与……显示出一些相当大的差异，并且用特殊的凝集素抑制毒素进行的进一步试验表明，不含其他辅助抗体的特异性抗毒素可能不具有凝集性，因此不能通过血凝抑制进行滴定。从……培养滤液中分离出的对磷酸盐稳定、对胃蛋白酶稳定和对胰蛋白酶稳定的抗原，被发现含有极少量的特异性类毒素，并且对这三种抗原制剂中的每一种进行交叉滴定表明，该组内其他抗原的污染极少。用分别用三种辅助抗原致敏的红细胞对白喉抗血清进行吸收试验，结果表明这些抗体具有高度特异性且各不相同。用三种不同抗原致敏的红细胞混合物对白喉抗血清进行吸收，可去除所有可检测到的辅助抗体，并且吸收后的血清不再能凝集用完整白喉类毒素致敏的细胞。然而，在滴定……时，吸收后的血清对白喉毒素仍保留大部分中和能力。通过血凝抑制对多种马血清中的每种辅助抗体进行滴定，结果表明，与絮状试验结果相比，对磷酸盐稳定抗原和胃蛋白酶稳定抗原的辅助抗体的值之间存在相关性，但与对胰蛋白酶稳定抗原的抗体的值无关。在包括多次重结晶步骤的滤液纯化的所有阶段，白喉毒素以及磷酸盐稳定和胃蛋白酶稳定抗原的相对比例保持恒定。然而，在该过程中，胰蛋白酶稳定抗原的浓度稳步下降。在合理的储存条件下，几种粗培养滤液中毒素、磷酸盐稳定和胃蛋白酶稳定抗原的相对比例是恒定的，而在发生变质的情况下则变化很大。这三种抗原在生长培养物中也保持恒定比例，而胰蛋白酶稳定抗原则不然。
