Hendrich S, Leveston A O, Pitot H C
Food Science and Human Nutrition, Iowa State University, Ames 50011.
Eur J Cancer Prev. 1992 Oct;1(6):407-14. doi: 10.1097/00008469-199210000-00003.
Female F344/N rats were given 70% partial hepatectomies and intubated with diethyl-nitrosamine (DEN, 10 mg/kg) 24 hours later. They were fed a cereal-based diet, NIH-07 (NIH) + 0.05% phenobarbital (PB) for 6 months, at which time NIH + PB was withdrawn and the rats were ovariectomized (OV) or sham-operated (SH). Groups of 7-10 rats were fed a semipurified diet (AIN-76) for 1 or 2 months after withdrawal of NIH + PB, or NIH + PB for 2 months, or AIN-76 diet for 1 month and subsequently NIH + PB for 1 month. Placental glutathione S-transferase (PGST)- and gamma-glutamyltransferase (GGT)-positive (+) altered hepatic foci (AHF) were analysed by quantitative stereology. Ovariectomy stimulated growth of AHF after withdrawal and reintroduction of NIH + PB. AHF, especially PGST+ AHF, continued to regress throughout the PB withdrawal period in rats fed AIN-76 diet. In most studies of chemical hepatocarcinogenesis, females have been shown to develop a greater volume of AHF than males. In our study, however, ovariectomy stimulated the growth of AHF after withdrawal and reintroduction of PB. Because AHF occurring spontaneously in male rats develop more rapidly than in female rats, the greater rate of growth of AHF in OV female rats may reflect a similar mechanism.
对雌性F344/N大鼠进行70%部分肝切除术，24小时后腹腔注射二乙基亚硝胺（DEN，10mg/kg）。给它们喂食以谷物为基础的饮食，即NIH-07（NIH）+0.05%苯巴比妥（PB），持续6个月，此时停止给予NIH+PB，并对大鼠进行卵巢切除术（OV）或假手术（SH）。在停止给予NIH+PB后，将7-10只大鼠分为一组，喂食半纯化饮食（AIN-76）1或2个月，或给予NIH+PB 2个月，或先给予AIN-76饮食1个月，随后给予NIH+PB 1个月。通过定量体视学分析胎盘谷胱甘肽S-转移酶（PGST）和γ-谷氨酰转移酶（GGT）阳性（+）的肝病灶（AHF）。卵巢切除术后，在停止给予并重新给予NIH+PB后，刺激了AHF的生长。在喂食AIN-76饮食的大鼠中，在整个停用PB期间，AHF，尤其是PGST+AHF持续消退。在大多数化学性肝癌发生的研究中，已表明雌性大鼠比雄性大鼠产生更大体积的AHF。然而，在我们的研究中，卵巢切除术后，在停止给予并重新给予PB后，刺激了AHF的生长。由于雄性大鼠自发出现的AHF比雌性大鼠发展得更快，OV雌性大鼠中AHF更高的生长速率可能反映了类似的机制。
