氧气对重组系统中亚硝酸盐还原的影响。

The influence of oxygen on nitrite reduction in a reconstituted system.

作者信息

Spiller H, Bookjans G, Böger P

出版信息

Z Naturforsch C Biosci. 1976 Sep-Oct;31(9-10):565-8. doi: 10.1515/znc-1976-9-1015.

DOI:10.1515/znc-1976-9-1015

Abstract

Data regarding the role of oxygen in nitrite reduction are presented. In an NADPH-generating system including homogeneously purified ferredoxin-NADP reductase, ferredoxin (or flavodoxin) and nitrite reductase from the alga Bumilleriopsis filiformis, oxygen and nitrite can be reduced simultaneously. In air, rates of 1.2 mumol nitrite reduced-min-1-mg-1 nitrite reductase are obtained, which are physiologically feasible. Ferredoxin is inhibited non-competitively by oxygen during nitrite reduction. Oxygen uptake due to the oxidase reaction of ferredoxin-NADP reductase mediated by flavodoxin from Chlorella fusca and ferredoxin from Bumilleriopsis involves superoxide and is inhibited by the nitrite reducing system.

摘要

本文介绍了有关氧气在亚硝酸盐还原过程中作用的数据。在一个产生NADPH的系统中，该系统包含来自丝状布氏藻的均一纯化的铁氧还蛋白-NADP还原酶、铁氧还蛋白（或黄素氧还蛋白）和亚硝酸盐还原酶，氧气和亚硝酸盐能够同时被还原。在空气中，可获得1.2 μmol亚硝酸盐还原·分钟⁻¹·毫克⁻¹亚硝酸盐还原酶的速率，这在生理上是可行的。在亚硝酸盐还原过程中，铁氧还蛋白受到氧气的非竞争性抑制。由小球藻的黄素氧还蛋白和丝状布氏藻的铁氧还蛋白介导的铁氧还蛋白-NADP还原酶的氧化酶反应所导致的氧气摄取涉及超氧化物，并且受到亚硝酸盐还原系统的抑制。