Romero L C, Gotor C, Márquez A J, Forde B G, Vega J M
Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Seville, Spain.
Biochim Biophys Acta. 1988 Nov 2;957(1):152-7. doi: 10.1016/0167-4838(88)90168-9.
Polyclonal antisera were prepared against ferredoxin-nitrite reductase (EC 1.7.7.1) and ferredoxin-glutamate synthase (glutamate synthase (ferredoxin); EC 1.4.7.1) from the green alga Chlamydomonas reinhardtii. The anti-glutamate synthase antibodies recognized both glutamate synthase and nitrite reductase, but inhibited only the ferredoxin-linked activity of the latter enzyme and not the activity dependent on methyl viologen. Analogously, the anti-nitrite reductase antibodies recognized glutamate synthase and nitrite reductase but the first enzyme was only poorly inhibited. Free ferredoxin protected the nitrite reductase against its inactivation by anti-glutamate synthase antibodies. These results indicate that the ferredoxin-dependent glutamate synthase and nitrite reductase from this alga share common antigenic determinants, and that these are located at the ferredoxin-binding domains.
针对莱茵衣藻的铁氧化还原蛋白-亚硝酸盐还原酶（EC 1.7.7.1）和铁氧化还原蛋白-谷氨酸合酶（谷氨酸合酶（铁氧化还原蛋白）；EC 1.4.7.1）制备了多克隆抗血清。抗谷氨酸合酶抗体识别谷氨酸合酶和亚硝酸盐还原酶，但仅抑制后者酶的铁氧化还原蛋白连接活性，而不抑制依赖甲基紫精的活性。类似地，抗亚硝酸盐还原酶抗体识别谷氨酸合酶和亚硝酸盐还原酶，但对第一种酶的抑制作用较弱。游离的铁氧化还原蛋白可保护亚硝酸盐还原酶不被抗谷氨酸合酶抗体灭活。这些结果表明，这种藻类中依赖铁氧化还原蛋白的谷氨酸合酶和亚硝酸盐还原酶具有共同的抗原决定簇，并且这些抗原决定簇位于铁氧化还原蛋白结合结构域。
