Lach H J, Böger P
Z Naturforsch C Biosci. 1976 Sep-Oct;31(9-10):606-11. doi: 10.1515/znc-1976-9-1021.
Cytochrome b6-f particles were prepared from chloroplasts of spinach and the heterokont alga Bumilleriopsis filiformis using digitonin in comparison with non-ionic detergents. The cytochrome b6-f particles are solubilized in different amounts according to chemical structure and HLB value of the detergent (see Griffin, J. Soc. Cosmet. Chem. 1, 311 [1949]). Also the ratio of the two cytochromes in the isolated particle depends on the detergent used. In case of spinach, it varies from Cyt b6:f=1.5:1 to 4.9:1. These investigations do not strengthen the idea that a cytochrome b6-f complex with a stoichiometric composition is present in the photosynthetic membrane. Application of different detergents is a means for selective solubilization of thylakoid membrane components.
使用洋地黄皂苷，并与非离子型去污剂作比较，从菠菜叶绿体和丝状异鞭藻制备了细胞色素b6-f颗粒。根据去污剂的化学结构和HLB值（见格里芬，《化妆品化学学会杂志》1，311 [1949]），细胞色素b6-f颗粒以不同的量被溶解。此外，分离颗粒中两种细胞色素的比例取决于所用的去污剂。就菠菜而言，其变化范围为细胞色素b6:f = 1.5:1至4.9:1。这些研究并未支持光合膜中存在化学计量组成的细胞色素b6-f复合物这一观点。使用不同的去污剂是选择性溶解类囊体膜成分的一种方法。
