Hurt E, Hauska G
Eur J Biochem. 1981 Jul;117(3):591-5. doi: 10.1111/j.1432-1033.1981.tb06379.x.
The isolation of a cytochrome f/b6 complex from spinach chloroplasts, with high yield and purity is reported. The complex consists of five polypeptides with a molecular mass of 34, 33, 23.5, 20 and 17.5 kDa, and contains one cytochrome f, two cytochromes b6 and the Rieske Fe-S center with two non-heme irons. It does not contain plastocyanin and is almost completely devoid of chlorophyll and carotenoids, but lipid and detergent are present. It is lacking cytochrome b-559, although three of the five polypeptides seem to carry heme groups. The preparation has plastoquinol-plastocyanin oxidoreductase activity with plastoquinol-1 and plastoquinol-9, which is sensitive to 2,5-dibromomethylisopropyl-p-benzoquinone, to 2-iodo-6-isopropyl-3-methyl-2',4',4'-trinitrodiphenyl ether, to 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole, and to bathophenanthroline. Characteristics of this activity with respect to substrate concentrations, pH, detergent effect and other parameters are described.
据报道，已从菠菜叶绿体中高产且纯地分离出细胞色素f/b6复合物。该复合物由分子量分别为34、33、23.5、20和17.5 kDa的五种多肽组成，含有一个细胞色素f、两个细胞色素b6以及带有两个非血红素铁的 Rieske Fe-S中心。它不含质体蓝素，几乎完全不含叶绿素和类胡萝卜素，但含有脂质和去污剂。它缺乏细胞色素b-559，尽管五种多肽中的三种似乎携带血红素基团。该制剂对质体醌-1和质体醌-9具有质体醌-质体蓝素氧化还原酶活性，对2,5-二溴甲基异丙基对苯醌、2-碘-6-异丙基-3-甲基-2',4',4'-三硝基二苯醚、5-正十一烷基-6-羟基-4,7-二氧苯并噻唑和邻二氮杂菲敏感。描述了该活性在底物浓度、pH、去污剂效应和其他参数方面的特征。
