Poller D N, Spendlove I, Baker C, Church R, Ellis I O, Plowman G D, Mayer R J
University Department of Histopathology, Queen's Medical Centre, Nottingham, U.K.
J Pathol. 1992 Nov;168(3):275-80. doi: 10.1002/path.1711680306.
A polyclonal rabbit antibody was raised to the c-erbB-3 protein using a synthetic peptide corresponding to amino acids 1229-1241 of the predicted protein sequence of c-erbB-3. In Western blot analysis this antibody detects a single band at approximately 165 kD in a c-erbB-3 transfected (293/HER-3) human cell line. c-erbB-3 protein expression was then examined in a variety of adenocarcinomas. Expression of c-erbB-3 protein was indicated by membrane and/or cytoplasmic tumour cell immunoreactivity in formalin-fixed, paraffin-embedded tissue sections. c-erbB-3 protein was detected in a series of 13 out of 14 primary breast carcinomas, 3 of 5 gastric adenocarcinomas, 8 of 9 colonic adenocarcinomas, 2 of 9 prostatic adenocarcinomas, 0 of 6 renal cell carcinomas, 1 of 4 primary lung adenocarcinomas, and 5 of 7 endometrial adenocarcinomas. Immunohistochemical expression of the c-erbB-3 protein appears to be a relatively common event in adenocarcinomas, and further studies are now warranted to establish the role of the c-erbB-3 protein in neoplasia.
利用对应于c-erbB-3预测蛋白序列第1229 - 1241位氨基酸的合成肽，制备了针对c-erbB-3蛋白的多克隆兔抗体。在蛋白质免疫印迹分析中，该抗体在c-erbB-3转染的（293/HER-3）人细胞系中检测到一条约165 kD的单一条带。然后在多种腺癌中检测c-erbB-3蛋白的表达。在福尔马林固定、石蜡包埋的组织切片中，膜和/或细胞质肿瘤细胞免疫反应性表明存在c-erbB-3蛋白表达。在14例原发性乳腺癌中有13例、5例胃腺癌中有3例、9例结肠腺癌中有8例、9例前列腺腺癌中有2例、6例肾细胞癌中有0例、4例原发性肺腺癌中有1例以及7例子宫内膜腺癌中有5例检测到c-erbB-3蛋白。c-erbB-3蛋白的免疫组化表达在腺癌中似乎是一个相对常见的现象，目前有必要进一步开展研究以确定c-erbB-3蛋白在肿瘤形成中的作用。
