Rajkumar T, Gooden C S, Lemoine N R, Gullick W J, Goden C S
Molecular Oncology Laboratory, Hammersmith Hospital, London, U.K.
J Pathol. 1993 Jul;170(3):271-8. doi: 10.1002/path.1711700309.
We have examined the expression of the c-erbB-3 protein in a wide range of tumours of the gastrointestinal (GI) tract using immunocytochemical staining. Two antibodies were employed, a polyclonal antibody, 49.3, and a new monoclonal antibody, RTJ1, both raised to a synthetic peptide from the cytoplasmic domain of the human c-erbB-3 protein. The antibodies recognize c-erbB-3 by immunoprecipitation and Western blotting of lysate prepared from a cell line engineered to overexpress the protein. Both antibodies also detect expression of the protein in formalin-fixed, paraffin-embedded human tissues. The RTJ1 monoclonal antibody gave superior staining, showing lower background and more pronounced cell surface immunoreactivity. The c-erbB-3 protein was found in normal epithelial cells throughout the GI tract, in squamous epithelium of the oropharanyx and oesophagus, in the parietal cells of the stomach, and in the surface enterocytes of the small and large bowel. Seventy-six tumours arising at these sites were examined for c-erbB-3 protein expression. Widely varying levels of expression were seen, from absent to intense staining with cell membrane accentuation.
我们运用免疫细胞化学染色法检测了多种胃肠道（GI）肿瘤中c-erbB-3蛋白的表达情况。使用了两种抗体，一种多克隆抗体49.3和一种新的单克隆抗体RTJ1，二者均针对人c-erbB-3蛋白胞质结构域的合成肽制备而成。这些抗体通过对经基因工程改造以过表达该蛋白的细胞系制备的裂解物进行免疫沉淀和蛋白质印迹法来识别c-erbB-3。两种抗体还能检测福尔马林固定、石蜡包埋的人体组织中该蛋白的表达。RTJ1单克隆抗体染色效果更佳，背景更低，细胞表面免疫反应性更强。在整个胃肠道的正常上皮细胞、口咽和食管的鳞状上皮、胃的壁细胞以及小肠和大肠的表面肠上皮细胞中均发现了c-erbB-3蛋白。对这些部位发生的76例肿瘤进行了c-erbB-3蛋白表达检测。观察到表达水平差异很大，从无表达到细胞膜强化的强烈染色。
