[The effect of acute poisoning of rats with carbaryl on the transamination reactions involving alpha-ketoglutarate. Part I. Activity of plasma aminotransferases].

White male Wistar rats were poisoned orally with a single carbaryl dose 474 mg/kg (1/2 LD50). In the plasma of control animals and 2, 4, 24 and 72 hours after administration of the insecticide the activity of aminotransferase catalysing the reactions between alpha-ketoglutarate and six amino acids (cysteine, lysine, phenylalanine, leucine, asparagine and valine) was determined. Aminotransferases activity was expressed as the amount of glutamic acid developing during incubation of 1 cm3 of plasma for 1 hour. Glutamic acid was determined spectrophotometrically after chromatographic separation on paper. The results of the investigations demonstrated that acute intoxication with carbaryl caused a statistically significant increase in the activity of two aminotransferases in the plasma of experimental animals. A rise in the activity of the enzyme catalysing the reaction between alpha-ketoglutarate and asparagine ketoglutarate and lysine was observed.