Boente M P, Berchuck A, Rodriguez G C, Davidoff A, Whitaker R, Xu F J, Marks J, Clarke-Pearson D L, Bast R C
Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC 27710.
Am J Obstet Gynecol. 1992 Dec;167(6):1877-82. doi: 10.1016/0002-9378(92)91790-h.
We examined the effect of interferon gamma on proliferation and epidermal growth factor receptor expression in ovarian cancer cell lines and normal ovarian epithelial cells.
The tritiated thymidine incorporation assay was used to assess the effect of interferon gamma on proliferation. Scatchard analysis of anti-epidermal growth factor receptor antibody binding, and Western blotting of immunoprecipitates was used to assess the effect of interferon gamma on epidermal growth factor receptor expression.
Although interferon gamma elicited 30% to 40% decreases in proliferation, epidermal growth factor receptor expression was strikingly increased in all four ovarian cancer cell lines. Scatchard analysis indicated that this increase occurred primarily at the cell surface, but total cellular receptor levels also were increased. In contrast, interferon gamma treatment of normal ovarian epithelial cells affected neither proliferation nor epidermal growth factor receptor levels.
Because the up-regulation of epidermal growth factor receptors by interferon gamma appears to be confined to malignant cells, interferon gamma may facilitate immunotherapy and imaging of ovarian cancers by means of immunoconjugates directed against the epidermal growth factor receptor.
我们研究了γ干扰素对卵巢癌细胞系和正常卵巢上皮细胞增殖及表皮生长因子受体表达的影响。
采用氚标记胸腺嘧啶核苷掺入试验评估γ干扰素对增殖的影响。通过对抗表皮生长因子受体抗体结合进行Scatchard分析以及对免疫沉淀物进行蛋白质印迹法来评估γ干扰素对表皮生长因子受体表达的影响。
尽管γ干扰素使增殖降低了30%至40%，但在所有四种卵巢癌细胞系中表皮生长因子受体表达显著增加。Scatchard分析表明这种增加主要发生在细胞表面，但细胞总受体水平也有所增加。相比之下，γ干扰素处理正常卵巢上皮细胞对增殖和表皮生长因子受体水平均无影响。
由于γ干扰素对表皮生长因子受体的上调似乎仅限于恶性细胞，γ干扰素可能通过针对表皮生长因子受体的免疫缀合物促进卵巢癌的免疫治疗和成像。
