Rodriguez G C, Berchuck A, Whitaker R S, Schlossman D, Clarke-Pearson D L, Bast R C
Department of Obstetrics and Gynecology, Duke University, Durham, NC.
Am J Obstet Gynecol. 1991 Mar;164(3):745-50. doi: 10.1016/0002-9378(91)90508-o.
Previously we have shown that epidermal growth factor acts as a mitogen for some, but not all, ovarian cancer cells in culture. In this study we examined the effect of epidermal growth factor on proliferation of normal human ovarian epithelial cells in monolayer culture. We found that epidermal growth factor stimulated twofold to fourfold increases in proliferation in epithelial cells from each of five normal ovaries (p less than 0.01). In addition, Scatchard analysis of binding of epidermal growth factor tagged with iodine 125 indicated the presence of high-affinity receptors in all of the ovarian epithelial cells and ovarian cancer cell lines. The number and affinity of receptors was similar in the normal epithelium and cancer cell lines, and there was no relationship between epidermal growth factor receptor number and responsiveness to epidermal growth factor. We conclude that human ovarian epithelial cells normally express epidermal growth factor receptors and that epidermal growth factor acts as a mitogen for these cells. Although the mitogenic response to epidermal growth factor often is attenuated in ovarian cancer cell lines, loss of responsiveness to epidermal growth factor does not appear to be due to decreased receptor expression.
此前我们已经表明，表皮生长因子在培养中对某些而非所有卵巢癌细胞起着促有丝分裂原的作用。在本研究中，我们检测了表皮生长因子对单层培养的正常人卵巢上皮细胞增殖的影响。我们发现，表皮生长因子刺激来自五个正常卵巢中每个卵巢的上皮细胞增殖增加了两倍至四倍（P<0.01）。此外，对用125碘标记的表皮生长因子结合进行的Scatchard分析表明，所有卵巢上皮细胞和卵巢癌细胞系中均存在高亲和力受体。正常上皮细胞和癌细胞系中受体的数量和亲和力相似，且表皮生长因子受体数量与对表皮生长因子的反应性之间没有关系。我们得出结论，人卵巢上皮细胞正常表达表皮生长因子受体，且表皮生长因子对这些细胞起着促有丝分裂原的作用。尽管卵巢癌细胞系中对表皮生长因子的促有丝分裂反应常常减弱，但对表皮生长因子反应性的丧失似乎并非由于受体表达减少所致。
