自身免疫性肠病患儿空肠黏膜中淋巴细胞功能相关抗原-1和细胞间黏附分子-1分子的表达

LFA-1 and ICAM-1 molecule expression in jejunal mucosa from children with autoimmune enteropathy.

作者信息

Hill S, Milla P J, Ciampolillo A, Napolitano G, Bottazzo G F, Mirakian R

机构信息

Department of Child Health, Institute of Child Health, London.

出版信息

Autoimmunity. 1992;13(3):233-41. doi: 10.3109/08916939209004829.

DOI:10.3109/08916939209004829

PMID:1361864

Abstract

The expression of adhesion molecules by cells of the small intestinal mucosa was compared in gut biopsies from children with autoimmune small intestinal enteropathy and normal controls and related to HLA-DR expression by the same tissue. Jejunal biopsies were stained by IFL with monoclonal antibodies to LFA-1 (TS1/22 and CD11a/25.3.1) and ICAM-1 (RR1/1 and 84H10) molecules. LFA-1 and ICAM-1 positive cells were observed in the lamina propria in all cases and the counts were increased in autoimmune enteropathy compared with controls. In addition, in 4 of 7 cases of autoimmune enteropathy crypt enterocytes were positives for ICAM-1 when stained with RR1/1 and 3 of the 4 were also positive for LFA-1 when stained with both LFA-1 reagents. We speculate on the role of adhesion molecule expression in autoimmune enteropathy.

摘要

对患有自身免疫性小肠病的儿童和正常对照者的肠道活检组织进行了比较，以研究小肠黏膜细胞黏附分子的表达情况，并将其与同一组织中 HLA-DR 的表达相关联。空肠活检组织用免疫荧光法（IFL），使用针对淋巴细胞功能相关抗原-1（LFA-1，TS1/22 和 CD11a/25.3.1）和细胞间黏附分子-1（ICAM-1，RR1/1 和 84H10）分子的单克隆抗体进行染色。在所有病例的固有层中均观察到 LFA-1 和 ICAM-1 阳性细胞，与对照组相比，自身免疫性肠病中的细胞计数增加。此外，在 7 例自身免疫性肠病病例中的 4 例中，隐窝肠上皮细胞在用 RR1/1 染色时 ICAM-1 呈阳性，在这 4 例中的 3 例在用两种 LFA-1 试剂染色时 LFA-1 也呈阳性。我们推测了黏附分子表达在自身免疫性肠病中的作用。