作用于Cl(-)-HCO3-和Na(+)-H+交换体的药物对大鼠胃黏膜片制备中酸分泌的影响。
Effects of drugs acting on Cl(-)-HCO3- and Na(+)-H+ exchangers on acid secretion in the rat gastric mucosa sheet preparation.
作者信息
Horie S, Yano S, Watanabe K
机构信息
Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
出版信息
Eur J Pharmacol. 1992 Dec 8;229(1):15-9. doi: 10.1016/0014-2999(92)90280-h.
The effects of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), an inhibitor of the Cl(-)-HCO3- exchanger, and amiloride, an inhibitor of the Na(+)-H+ exchanger, on gastric acid secretion under basal conditions and after stimulation with bethanechol or dibutyryl cyclic AMP were studied in rat gastric mucosa sheet preparation. DIDS inhibited bethanechol-induced acid secretion in a dose-dependent manner, but amiloride had no effect. The stimulation of acid secretion by dibutyryl cyclic AMP plus 3-isobutyl-1-methylxanthine was also inhibited by DIDs, but not by amiloride. DIDS did not reduce basal acid secretion, and neither did amiloride. These results suggest that the Cl(-)-HCO3-exchanger in the basolateral membrane of the parietal cell plays an important role in stimulated gastric acid secretion and that the Na(+)-H+ exchanger is less important. In addition, these data show that DIDS inhibits stimulated gastric acid secretion irrespective of the secretagogue, but not basal gastric acid secretion.
在大鼠胃黏膜片制备中，研究了氯离子-碳酸氢根离子交换体抑制剂4,4'-二异硫氰基芪-2,2'-二磺酸（DIDS）和钠离子-氢离子交换体抑制剂氨氯吡咪对基础状态下以及用氨甲酰甲胆碱或二丁酰环磷腺苷刺激后的胃酸分泌的影响。DIDS以剂量依赖方式抑制氨甲酰甲胆碱诱导的胃酸分泌，但氨氯吡咪无此作用。二丁酰环磷腺苷加3-异丁基-1-甲基黄嘌呤对胃酸分泌的刺激也被DIDS抑制，但未被氨氯吡咪抑制。DIDS不降低基础胃酸分泌，氨氯吡咪也不降低。这些结果表明，壁细胞基底外侧膜中的氯离子-碳酸氢根离子交换体在刺激的胃酸分泌中起重要作用，而钠离子-氢离子交换体不太重要。此外，这些数据表明，DIDS抑制不论何种促分泌剂刺激的胃酸分泌，但不抑制基础胃酸分泌。
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