小鼠肝线粒体中p32摄取的体外研究。
In vitro studies of p32 uptake in mouse liver mitochondria.
作者信息
EDMUNDS A B
出版信息
J Gen Physiol. 1959 Jan 20;42(3):579-87. doi: 10.1085/jgp.42.3.579.
Isolated mouse liver mitochondria were incubated in two types of P(32)-labelled sucrose-phosphate buffers. The first contained no added ATP or oxidizable substrate. The second contained added ATP. Samples were taken at specified times, up to 60 minutes, and analyses were made of the mitochondrial TCA-soluble inorganic P(32) and the total mitochondrial residue P(31) and P(32). The results of the analyses showed that when the phosphorus inhibition index (the ratio of the amount of incubation inorganic phosphorus to the square of the amount of tyrosine in the mitochondria) was high, inorganic P(32) uptake was low and vice versa. In accordance with established data, increased P(32) uptake was obtained when ATP was added. ATP was found to stabilize the turnover of TCA-insoluble residue phosphorus as well as to maintain the TCA-soluble orthophosphate pool. These results support findings regarding the inhibitory and controlling effects of incubation medium phosphate in the regulation of inorganic phosphorus uptake.
将分离出的小鼠肝脏线粒体置于两种含磷（32）的蔗糖 - 磷酸盐缓冲液中进行孵育。第一种缓冲液中未添加ATP或可氧化底物。第二种缓冲液中添加了ATP。在长达60分钟的特定时间点采集样本，并对线粒体三羧酸循环（TCA）可溶性无机磷（32）以及线粒体总残留磷（31）和磷（32）进行分析。分析结果表明，当磷抑制指数（孵育无机磷量与线粒体中酪氨酸量的平方之比）较高时，无机磷（32）摄取量较低，反之亦然。根据已有的数据，添加ATP时可使磷（32）摄取量增加。发现ATP可稳定三羧酸循环不溶性残留磷的周转，同时维持三羧酸循环可溶性正磷酸盐池。这些结果支持了关于孵育培养基磷酸盐在调节无机磷摄取中的抑制和控制作用的研究发现。
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