Ohagi S, Ekawa K, Sanke T, Nishi M, Nanjo K
First Department of Medicine, Wakayama University of Medical Science, Japan.
Diabetes Res Clin Pract. 1992 Nov;18(2):71-4. doi: 10.1016/0168-8227(92)90001-8.
Two restriction fragment length polymorphisms (RFLPs) near the human islet amyloid polypeptide (IAPP) gene were examined in 50 Japanese patients with non-insulin-independent diabetes mellitus (NIDDM) and 54 non-diabetic controls. RFLPs were identified with the enzymes PvuII (A1 = 21 kb and A2 = 18 kb) and BglII (B1 = 9 kb and B2 = 7 kb). These RFLPs were in complete linkage disequilibrium with A1 which was in disequilibrium with B2, as was A2 with B1. Since these two RFLPs map to different locations in the 5'-flanking region of the IAPP gene, they are most likely due to changes in the sequence of the sites recognized by PvuII and BglII rather than to an insertion/deletion-type DNA polymorphism. There were no differences in the genotypic or allelic frequencies of these RFLPs between Japanese subjects with NIDDM and non-diabetic controls implying that these RFLPs do not play a major role in the development of NIDDM in this population.
在50名日本非胰岛素依赖型糖尿病（NIDDM）患者和54名非糖尿病对照者中，检测了人胰岛淀粉样多肽（IAPP）基因附近的两种限制性片段长度多态性（RFLP）。用PvuII酶（A1 = 21 kb和A2 = 18 kb）和BglII酶（B1 = 9 kb和B2 = 7 kb）鉴定RFLP。这些RFLP与A1完全连锁不平衡，A1与B2不平衡，A2与B1也不平衡。由于这两种RFLP定位于IAPP基因5'侧翼区的不同位置，它们很可能是由于PvuII和BglII识别位点的序列变化，而不是由于插入/缺失型DNA多态性。在日本NIDDM患者和非糖尿病对照者之间，这些RFLP的基因型或等位基因频率没有差异，这意味着这些RFLP在该人群NIDDM的发生中不发挥主要作用。
